NDLI: Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus

Content Provider	Springer Nature Link
Author	Wu, Jin Hu Miller, Shirley A. Hall, Harvey K. Mooney, Pauline A.
Copyright Year	2009
Abstract	Factors affecting micropropagation efficiency of 32 selections of Rubus, including the pre-treatment and initiation culture stages, were investigated. Chilling at 4°C for 6 weeks as a pre-treatment significantly promoted in vitro initiation culture; up to 65% of initiation cultures post chilling were successful. The cytokinin 6-benzyladenine (BA) was the most effective of the three tested for promotion of multiple shoot development in culture, with an average of three to seven shoots or plantlets developed from each single node. Alternating the concentration of BA between 4.44 and 13.31 μM from sub-culture to sub-culture improved recalcitrant Rubus micropropagation, and reduced the problems associated with long-term culture in the presence of high concentrations of BA. Reduction of the strength of macro- and micro-elements in the basal medium from half to one-third, with addition of 0.49 μM indole-3-butyric acid and 0.05% activated charcoal, and placing the cultures under reduced light intensity (17 μmol m$^{−2}$ s$^{−1}$), was found to alleviate chlorosis and improve micropropagation with high quality Rubus plantlets. Response to in vitro culture differed greatly and consequently different methods of micropropagation were required by different genotypes. From these selections, three types of micropropagation including micro-cutting, micro-shoots and multi-shoots were observed, and their efficiency was characterized.
Starting Page	17
Ending Page	25
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	99
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-08-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	In vitro propagation Activated charcoal Alternate culture Micro-cutting Micro-shoots Multi-shoots Raspberry Black raspberry Hybridberry Boysenberry Thimbleberry Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

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