NDLI: Effect of TDZ and 2, 4-D on peanut somatic embryogenesis and in vitro bud development

Content Provider	Springer Nature Link
Author	Joshi, Madhumita Sujatha, K. Hazra, Sulekha
Copyright Year	2008
Abstract	Failure of peanut somatic embryos to convert into plantlets is attributed to the abnormal development of the plumule. Thidiazuron (TDZ) was effective in the conversion of peanut somatic embryos to plantlets by triggering morphogenetic activity in the abnormal plumules of the rooted somatic embryos. The present study aimed to induce normal embryo differentiation by culturing the embryogenic masses in embryo development medium containing 2,4-D and various concentrations of TDZ. Although this was not achieved due to restricted somatic embryo development in the presence of TDZ, bud-like projections appeared in the embryogenic masses when these were cultured in media containing combinations of 2,4-D and TDZ. These projections developed into buds, which subsequently formed shoots and plantlets. The response varied with the concentration and exposure of TDZ. At lower concentrations, the buds appeared in a defined row in the equatorial region of the explant, and with extended incubation, more and more buds appeared in rows alongside the initial row. Induction of multiple buds in a defined row in this specific site (equatorial region) suggested the presence of potent cells around this region. At higher concentrations, these projections appeared in large numbers spread over the whole upper part of the embryogenic mass starting from the equatorial region. The ability of embryogenic mass to convert into organogenic mass and to produce large number of organogenic buds provides an excellent system for basic studies and for the genetic transformation of peanut.
Starting Page	85
Ending Page	90
Page Count	6
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	94
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-17
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arachis hypogaea Embyogenic mass Somatic embryogenesis Thidiazuron Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The role of proline in thidiazuron-induced somatic embryogenesis of peanut

Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.)

In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Somatic embryogenesis from the axillary meristems of peanut (Arachis hypogaea L.)

Somatic embryogenesis of Merwilla plumbea (Lindl.) Speta

Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea.

Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis

TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2,4-D

Effect of TDZ and 2, 4-D on peanut somatic embryogenesis and in vitro bud development

Similar Documents

The role of proline in thidiazuron-induced somatic embryogenesis of peanut

Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.)

In vitro plant regeneration of Arachis correntina (Leguminosae) through somatic embryogenesis and organogenesis

An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Somatic embryogenesis from the axillary meristems of peanut (Arachis hypogaea L.)

Somatic embryogenesis of Merwilla plumbea (Lindl.) Speta

Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea.

Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis

TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2,4-D

Effect of TDZ and 2, 4-D on peanut somatic embryogenesis and in vitro bud development