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| Content Provider | Springer Nature Link |
|---|---|
| Author | Yu, Bo Liao, Feixiong Liu, Jinmei Sun, Yingbo Huang, Lili |
| Copyright Year | 2016 |
| Abstract | An efficient and stable transformation system was developed based on embryogenic cell suspension culture by Agrobacterium tumefaciens-mediated method in Spathiphyllum cannifolium. Embryogenic calli induced from leaves on MS medium with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L thidiazuron were incubated in liquid medium with identical growth regulators for suspension cultivation. The plantlet regeneration rate of embryogenic suspension cell aggregates (ESCAs) incubated on MS medium achieved 100 %. The ESCAs were transformed using A. tumefaciens strain EHA105 harboring a vector pCAMBIA1301 containing the β-glucuronidase (gusA) reporter gene and the hygromycin phosphotransferase II (hpt II) selection marker gene. The optimized transformation conditions were as follows: infiltration of ESCAs with Agrobacterium for 10 min under 60 kPa vacuum pressure, followed by co-cultivation on medium containing 200 μM acetosyringone for 3 days. In total, 858 plants were regenerated from 1200 infected ESCAs. Analyses of randomly selected regenerated plants by GUS assays, PCR and dot blot showed that 91.30 % were transgenic. Southern blot analyses revealed that the copy number of T-DNAs integrated into the genome ranged from one to three. This newly developed transformation system provides a basis for the future development of genetic engineering techniques to improve flower color and enhance the disease resistance of peace lily. |
| Starting Page | 325 |
| Ending Page | 334 |
| Page Count | 10 |
| File Format | |
| ISSN | 01676857 |
| Journal | Plant Cell, Tissue and Organ Culture (PCTOC) |
| Volume Number | 127 |
| Issue Number | 2 |
| e-ISSN | 15735044 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2016-07-30 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Spathiphyllum cannifolium Embryogenic suspension cultivation Plant regeneration Agrobacterium tumefaciens Transformation Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Horticulture |
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