NDLI: Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium

Content Provider	Springer Nature Link
Author	Staikidou, Ioanna Watson, Sally Harvey, Barbara M. R. Selby, Christopher
Copyright Year	2005
Abstract	Shoot clump cultures of Narcissus cultivars St. Keverne and Hawera were used to investigate the effects of culture medium carbon supply, type of carbohydrate and osmolarity on in vitro bulblet development. Increasing the medium osmolarity using mannitol or sorbitol, which did not act as substrates for growth, failed to stimulate bulblet formation with either cultivar. An exception to this was a relatively small increase in total bulblet dry weight per culture, in the cultivar Hawera only, caused by adding 30 g l $^{−1}$ sorbitol in combination with 30 g l$^{−1}$ sucrose. Simultaneously increasing the medium osmolarity and carbon supply using the metabolisable carbohydrate sources, sucrose, glucose, fructose or an equimolar mixture of glucose and fructose stimulated bulblet production, total dry matter accumulation and partitioning into bulblets. At comparable levels of carbon supply up to 19.0 g l$^{−1}$, bulblet development of both cultivars was similar with monosaccharide and sucrose media. This indicates that substrate supply is more important for bulblet development than osmolarity of the culture medium. The cultivar Hawera also showed similar responses to monosaccharide and sucrose media supplying 37.9 g C l$^{−1}$, despite the high osmolarity of monosaccharide media (c. 650 m Osm kg$^{−1}$, equivalent to −1.6 MPa, compared to 380 m Osm kg$^{−1}$ for sucrose medium). However in St. Keverne total dry matter accumulation and dry weight per bulblet were further stimulated only by increasing the sucrose supply from 19.0 to 37.9 g C l$^{−1}$, not by increasing the monosaccharide supply. Implications of the findings for Narcissus micropropagation are discussed.
Starting Page	313
Ending Page	320
Page Count	8
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	80
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	bulb cultivar effects micropropagation monosaccharides sucrose Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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