NDLI: Effect of nitrogen supply on expression of some genes controlling storage proteins and carbohydrate synthesis in cultured maize kernels

Content Provider	Springer Nature Link
Author	Doehlert, Douglas C. Duke, Edwin R. Smith, Leslie J.
Copyright Year	1997
Abstract	Maize kernels (Zea mays L., inbred W64A) were culturedin vitro with a 200 mM sucrose concentration and either 0, 3.6, 7.1, 14.3, or 36.5 mM N, supplied as amino acids, in the culture medium. Increased N supply up to 14.3 mM N resulted in increased kernel dry weight and starch content. Increasing N supply increased storage protein 3-fold, which was reflected by 7-fold enhancement ofα-zein message abundance.β-Zein message abundance was not affected by N nutrition. Messages for the regulatory gene,Opaque-2, were doubled by the increased N supply. Messages for theShrunken, Waxy, andAldolase genes increased about 2-fold with increased N supply. Activities of sucrose synthase (encoded byShrunken), and aldolase enzymes exhibited increases with N supply corresponding with that of their transcript abundance. Expression of theShrunken-2 andBrittle-2 genes were not affected by N supply. It is likely that the increased expression of genes involved in carbohydrate metabolism is responsible for the increased capacity of a kernel to utilize available sucrose at higher levels of N nutrition.
Starting Page	195
Ending Page	198
Page Count	4
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	47
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	corn enzyme activities kernel culture Zea mays zein Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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