NDLI: Physiological responses to salt stress of T2 alfalfa progenies carrying a transgene for betaine aldehyde dehydrogenase

Content Provider	Springer Nature Link
Author	Yan, Li Ping Liu, Cui Lan Liang, Hui Min Mao, Xiu Hong Wang, Fei Pang, Cai Hong Shu, Jing Xia, Yang
Copyright Year	2011
Abstract	Glycinebetaine is an important quaternary ammonium compound generated in response to salt and other osmotic stresses in many organisms. Its synthesis requires the catalysis of betaine aldehyde dehydrogenase encoded by a Betaine Aldehyde Dehydrogenase (BADH) gene that converts betaine aldehyde into glycinebetaine in some halotolerant plants. In this study, a BADH gene was over expressed in transgenic alfalfa (Medicago sativa L) plants using Agrobacterium-mediated transformation. Transgenic alfalfa plants grown under 9‰ NaCl grew well; while non-transgenic control plants turned yellowish in color, wilted, and eventually died. Polymerase chain reaction (PCR) and Northern blot hybridization analyses demonstrated that the BADH gene was transferred into the T2 generation and segregated in a Mendelian fashion. Transgenic alfalfa plants expressing BADH showed significantly higher BADH enzyme activity and betaine contents when grown under 6‰ NaCl. Moreover, proline content in T2 lines were higher while electrolyte leakage and malonaldehyde content were lower in T2 lines compared with non-transgenic plants. These findings indicated that transgenic plants expressing BADH transgene exhibited higher salt tolerance than non-transgenic plants.
Starting Page	191
Ending Page	199
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	108
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BADH gene Transgenic alfalfa Salt tolerance Plant Pathology Plant Physiology Plant Sciences Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Horticulture

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