NDLI: An improved procedure for protoplast microelectrofusion and culture of Nicotiana tabacum intraspecific somatic hybrids: plant regeneration and initial proofs on organelle segregation

Content Provider	Springer Nature Link
Author	Rákosy Tican, Lenuta Hork, Maria Menczel, Lászlo
Copyright Year	2001
Abstract	Somatic hybrids were produced between haploid Nicotiana tabacum L. cv. Petite Havana (wild type) and haploid streptomycin resistant (SR1) mutant by an improved version of microelectrofusion of preselected pairs of protoplasts and the culture of fusion products in a nurse culture. Resistance of diploid plants regenerated from 20 somatic hybrid clones was tested at low concentration of streptomycin in the light as well as at high concentrations of streptomycin in the dark. In two independent hybrid lines, plants resistant in the light but sensitive in the dark were found. The existence of this plant type indicates a segregation of chloroplasts and mitocondria in somatic hybrid clones. It is suggested that microelectrofusion of preselected pairs of protoplasts combined with a reliable nurse culture might be a good technique for controlled somatic hybridization, cell reconstitution and partial gene transfer to different plant species. It might also be used to follow and analyse organelle segregation in somatic hybrid clones. The possibility that mitochondria might be resistant to streptomycin in the SR1 mutant is also discussed.
Starting Page	153
Ending Page	158
Page Count	6
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	67
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Regeneration of somatic hybrids of ginger via chemical protoplast fusion

Protoplast isolation and culture for somatic hybridization of Lupinus angustifolius and L. subcarnosus

Redox and hormone profiling of a Nicotiana tabacum dedifferentiated protoplast culture suggests a role for a cytokinin and gibberellin in plant totipotency

The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle)

Transmission of organelle genomes in citrus somatic hybrids

Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis

Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum)

Production of phosphohydrolases by Nicotiana tabacum 1507 cell suspension culture

Production of somatic hybrids between Diospyros glandulosa and D. kaki by protoplast fusion

An improved procedure for protoplast microelectrofusion and culture of Nicotiana tabacum intraspecific somatic hybrids: plant regeneration and initial proofs on organelle segregation

Similar Documents

Regeneration of somatic hybrids of ginger via chemical protoplast fusion

Protoplast isolation and culture for somatic hybridization of Lupinus angustifolius and L. subcarnosus

Redox and hormone profiling of a Nicotiana tabacum dedifferentiated protoplast culture suggests a role for a cytokinin and gibberellin in plant totipotency

The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle)

Transmission of organelle genomes in citrus somatic hybrids

Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis

Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum)

Production of phosphohydrolases by Nicotiana tabacum 1507 cell suspension culture

Production of somatic hybrids between Diospyros glandulosa and D. kaki by protoplast fusion

An improved procedure for protoplast microelectrofusion and culture of Nicotiana tabacum intraspecific somatic hybrids: plant regeneration and initial proofs on organelle segregation