NDLI: CO$_{2}$-enriched atmosphere and supporting material impact the growth, morphophysiology and ultrastructure of in vitro Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen] plantlets

Content Provider	Springer Nature Link
Author	Saldanha, Cleber Witt Otoni, Caio Gomide Rocha, Diego Ismael Cavatte, Paulo Cézar Detmann, Kelly da Silva Coutinho Tanaka, Francisco André Ossamu Dias, Leonardo Lucas Carnevalli DaMatta, Fábio Murilo Otoni, Wagner Campos
Copyright Year	2014
Abstract	This study aimed to evaluate under photoautotrophic conditions the effect of CO$_{2}$-enriched atmosphere (360 or 1,000 μmol CO$_{2}$ mol$^{−1}$ air) combined with two substrate types (agar or Florialite$^{®}$) in vitro on plants of Pfaffia glomerata, an endangered medicinal species with promising applications in phytotherapy and phytomedicine. The effects of the treatments on the growth, stomatal density, Rubisco activity, carbon isotopic discrimination, metabolite accumulation, photosynthetic pigments and ultrastructural characteristics were investigated. After a 35-day cultivation period, the in vitro-growth of P. glomerata nodal segments under the different treatments resulted in plants with substantial differences in relation to their growth, photosynthetic pigments, stomatal density and leaf ultrastructural characteristics. The enrichment with CO$_{2}$ coupled with a porous substrate increased the growth of P. glomerata. The stomatal density in the abaxial epidermis more than doubled in response to the high CO$_{2}$ supply in both supporting types, whereas the Rubisco activity and activation state were both unresponsive to the treatments. Regardless of the CO$_{2}$ supply, the plants grown in agar displayed higher carbon isotope discrimination than their counterparts grown in Florialite$^{®}$. We propose that the long-term photosynthetic performance was improved using Florialite$^{®}$ as a growth support in combination with a high CO$_{2}$ supply. No apparent signs of photosynthetic down-regulation could be found under elevated CO$_{2}$ conditions. The enrichment of in vitro atmospheres with CO$_{2}$ coupled with a porous substrate offers new possibilities for improving the growth and production on a commercial scale of high morphological and physiological quality Pfaffia plants.
Starting Page	87
Ending Page	99
Page Count	13
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	118
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-03-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	β-Ecdysone Carbon isotopic discrimination Photoautotrophic growth Porous substrate metabolites Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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