NDLI: Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

Content Provider	Springer Nature Link
Author	Grover, Abhinav Yadav, Jayashankar S. Biswas, Ranjita Pavan, Choppakatla S. S. Mishra, Punita Bisaria, Virendra S. Sundar, Durai
Copyright Year	2011
Abstract	Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS or Heller medium containing different levels of 6-benzyladenine (BA) (0.05–2 mg l$^{−1}$), 2,4-dichlorophenoxyacetic acid (2,4-D) (1–10 mg l$^{−1}$), and sucrose (10–50 g l$^{−1}$). Moreover, the pH of the medium was varied from 5.2–6.2. In addition, cultures were subjected to light irradiation as well as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major monoterpenoids obtained were α-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol, benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds were obtained from cell suspension cultures grown in MS medium containing 5 mg l$^{−1}$ 2,4-D, 1 mg l$^{−1}$ BA and 30 g l$^{−1}$ sucrose at pH of 5.8 with incubation in complete darkness.
Starting Page	323
Ending Page	331
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	108
Issue Number	2
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Camellia sinensis cell suspension cultures Monoterpenoids Nerol Tea α-terpineol Plant Pathology Plant Physiology Plant Sciences Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Horticulture

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