NDLI: Somatic embryogenesis and regeneration from shoot primordia of Crocus heuffelianus

Content Provider	Springer Nature Link
Author	Demeter, Zita Surányi, Gyula Molnár, V. Attila Sramkó, Gábor Beyer, Dániel Kónya, Zoltán Vasas, Gábor M Hamvas, Márta Máthé, Csaba
Copyright Year	2009
Abstract	Crocus heuffelianus belongs to the C. vernus (Iridaceae) species aggregate. In the Carpathian Basin and particularly in Hungary it is considered an endangered species. Therefore our aim was to establish a tissue culture system with potential of germplasm preservation of this taxon. For in vitro culture experiments, shoot primordia from corms were the most suitable. We induced an embryogenic callus line from those explants on basal Murashige-Skoog (MS) medium supplemented with Gamborg’s vitamins, 2% (w/v) sucrose, 10 mg l$^{−1}$ (53.7 μM) α-naphthaleneacetic acid (NAA) and 1 mg l$^{−1}$ (4.44 μM) 6-benzyladenine (BA). Globular stage embryos developed on this medium and several culture conditions were used in an attempt to obtain mature embryos and plant regeneration. Firstly a decrease of auxin/cytokinin concentration and ratio, then secondly a decrease in the strength of culture medium and the concentration of carbon source was used, which was effective in embryogenesis and the production of plants. Regeneration medium used in the second step was fourfold diluted MS medium and Gamborg’s vitamins supplemented with 1% (w/v) sucrose, 0.05 mg l$^{−1}$ (0.26 μM) NAA and 0.5 mg l$^{−1}$ (2.22 μM) BA, with a 14/10 h photoperiod. Under these conditions we could detect all the stages of somatic embryo development characteristic for Iridaceae. This is the first report demonstrating the production of stable tissue culture of C. heuffelianus with potential use in germplasm preservation via plant regeneration. This study could also contribute to a better understanding of somatic embryogenesis in the Crocus genus.
Starting Page	349
Ending Page	353
Page Count	5
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	100
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-12-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Crocus heuffelianus Mature somatic embryo Plant regeneration Germplasm preservation Plant Pathology Plant Genetics & Genomics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum

The effect of auxins, time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat

Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes

Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

Somatic embryogenesis and plantlet regeneration from mature zygotic embryos of Manchurian ash (Fraxinus mandshurica Rupr.)

Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Somatic embryogenesis and plant regeneration in zygotic embryos of Trifolium nigrescens (Viv.)

Somatic embryogenesis and regeneration from shoot primordia of Crocus heuffelianus

Similar Documents

Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum

The effect of auxins, time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat

Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes

Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

Somatic embryogenesis and plantlet regeneration from mature zygotic embryos of Manchurian ash (Fraxinus mandshurica Rupr.)

Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai

Somatic embryogenesis induction in leaves and petioles of a mature wild olive

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Somatic embryogenesis and plant regeneration in zygotic embryos of Trifolium nigrescens (Viv.)

Somatic embryogenesis and regeneration from shoot primordia of Crocus heuffelianus