NDLI: Molecular analysis on the shoot apical meristem of truncated leaf syndrome plantlets of oil palm (E. guineensis Jacq.)

Content Provider	Springer Nature Link
Author	Habib, S. H. Ho, C. L. Syed Alwee, S. S. R. Namasivayam, P.
Copyright Year	2014
Abstract	Truncated leaf syndrome (TLS) is a vegetative somaclonal variant of oil palm that is regenerated from cultured somatic cells. Until now, there are no molecular clues associated to this abnormality. Forward and reverse suppressive subtractive hybridization (SSH) libraries were constructed to isolate the differentially expressed genes from the shoot apical meristem (SAM) of severe TLS plantlets. From the suppressive SSH libraries, 278 and 132 transcripts were up-regulated and down-regulated respectively in severe TLS SAM. The annotated 54 and 24 % unique gene sequences from the forward and reverse libraries had significant matches with known proteins and were further classified based on their putative functions. Real time RT-PCR indicated that six transcripts, namely a putative finger transcription factor, a Ring H2 Finger Protein, a putative far-red impaired response protein, a Pescadillo N-terminus family protein, a TLD family protein and a maternal effect embryo arrest 14 protein from the forward library were expressed at higher levels in the severe and moderate TLS SAM and at a lower level in the mild TLS SAM compared to the calibrator (normal SAM). Four transcripts that are hypothetical protein-1, hypothetical protein-2, catalase and cation transporter from the reverse library exhibited lower expression levels in the severe and moderate TLS shoot apical meristem compared to the calibrator (normal SAM). The present findings provide a basic molecular knowledge on some of the genes associated to the TLS syndrome in oil palm. However, it is unclear whether upregulation or downregulation of these genes are a consequence of TLS or the cause of TLS.
Starting Page	1023
Ending Page	1036
Page Count	14
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	120
Issue Number	3
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-11-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oil palm Somaclonal variant TLS Abnormality Tissue culture Gene expression Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant

Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

Ex Vitro Rooting of Oil Palm (Elaeis Guineensis Jacq.) Plantlets Derived From Tissue Culture

A candidate auxin-responsive expression marker gene, EgIAA9, for somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Tissue and temporal expression of miR172 paralogs and the AP2-like target in oil palm (Elaeis guineensis Jacq.)

EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Differential responses to somatic embryogenesis of different genotypes of Brazilian oil palm (Elaeis guineensis Jacq.)

Biotization of in vitro calli and embryogenic calli of oil palm (Elaeis guineensis Jacq.) with diazotrophic bacteria Herbaspirillum seropedicae (Z78)

Molecular analysis on the shoot apical meristem of truncated leaf syndrome plantlets of oil palm (E. guineensis Jacq.)

Similar Documents

Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant

Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

Ex Vitro Rooting of Oil Palm (Elaeis Guineensis Jacq.) Plantlets Derived From Tissue Culture

A candidate auxin-responsive expression marker gene, EgIAA9, for somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Tissue and temporal expression of miR172 paralogs and the AP2-like target in oil palm (Elaeis guineensis Jacq.)

EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Differential responses to somatic embryogenesis of different genotypes of Brazilian oil palm (Elaeis guineensis Jacq.)

Biotization of in vitro calli and embryogenic calli of oil palm (Elaeis guineensis Jacq.) with diazotrophic bacteria Herbaspirillum seropedicae (Z78)

Molecular analysis on the shoot apical meristem of truncated leaf syndrome plantlets of oil palm (E. guineensis Jacq.)