NDLI: Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase

Content Provider	Springer Nature Link
Author	Nyíri, Kinga Papp Kádár, Veronika Szabó, Judit E. Németh, Veronika Vértessy, Beáta G.
Copyright Year	2015
Abstract	dUTPases are essential for maintaining genome integrity. Recently, in the case of a dUTPase from a Staphylococcal phage, another different physiological function was also suggested. Namely, it was shown that dUTPase from the Staphylococcus aureus bacteriophage Ф11 is capable of binding to the Staphylococcal Stl repressor protein. This binding interferes with the function of Stl. In the present study, we investigated the putative role of a phage dUTPase-specific peptide segment in the interaction of dUTPase with Stl and in impeding Stl–DNA complex formation. We show that dUTPase from Mycobacterium tuberculosis that lacks the phage-specific insert is also capable of disrupting the complexation between Stl and DNA. Hence, the insert segment is not essential for perturbation of the Stl function. However, we also demonstrate that in case of the phage dUTPase, the insert-lacking construct is deficient in perturbation of Stl activity. These findings clearly indicate that the phage-specific insert has a well-defined role only in the context of the phage dUTPase.
Starting Page	1425
Ending Page	1432
Page Count	8
File Format	PDF
ISSN	10400400
Journal	Structural Chemistry
Volume Number	26
Issue Number	5-6
e-ISSN	15729001
Language	English
Publisher	Springer US
Publisher Date	2015-08-30
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	dUTPase Non-canonical insert Protein–protein interaction Protein–DNA interaction Staphylococcal repressor Computer Applications in Chemistry Physical Chemistry Theoretical and Computational Chemistry
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Condensed Matter Physics

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Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage φ11

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Exploring the role of the phage-specific insert of bacteriophage Φ11 dUTPase

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