NDLI: Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro

Content Provider	Springer Nature Link
Author	Smetanina, I. G. Tatariva, L. V. Krivokharchenko, A. S.
Copyright Year	2000
Abstract	We studied the capacity of the cattle oocyte for the resumption of meiosis and the achievement of metaphase II in various protein-free culture media (DMEM, TCM-199, Ham’s F-10, and Ham’s F-12) and the pattern of influence of the estrous serum on thein vitro development of fertilized cattle oocytes, with special reference to the time of its addition to the synthetic oviduct fluid containing BSA. In the first experimental series, it was shown that the highest number of oocytes (76.1%) resumed meiosis in DMEM medium. Meiosis was not resumed in Ham’s F-12. Intermediate results were obtained for TCM-199 (55.1%), which is commonly used for the maturation of cattle oocytesin vitro and for Ham’s F-10 (51.7%). The oocytes reached metaphase II in DMEM at a higher rate (45.3%) than in TCM-199 or Ham’s F-10 (29.4 and 8.6%, respectively). In the second experimental series, the estrous serum was added to the culture medium within 20 h (control) or 42 h (experiment) after the beginning of fertilization. The estrous serum did not inhibit the first cleavage division (the percentage of cleaving embryos did not differ reliably: 32.7 and 37.9%, respectively). However, a later serum addition to the culture medium (within 42 h after the beginning of fertilization) reliably increased the percentage of embryos that reached the blastocyst stage (6.5% in the control and 17.8% in the experiment) and the hatched blastocyst stage (2 and 9.2%, respectively).
Starting Page	113
Ending Page	116
Page Count	4
File Format	PDF
ISSN	10623604
Journal	Russian Journal of Developmental Biology
Volume Number	31
Issue Number	2
e-ISSN	16083326
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2000-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	preimplantation embryos oocytes cultivationin vitro cattle Developmental Biology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Developmental Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

In vitro fertilization of bovine oocytes in protein-free culture system

Nonhormonal stimulation in vitro of oocyte maturation in sturgeons

Influence of culture medium osmolality on maturation and ovulation of common frog oocytes stimulated in vitro by pituitary extract or progesterone

Traditional and modern approaches to culture of preimplantation mammalian embryos in vitro

Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

Effects of NO-synthase inhibitors on maturation mouse oocytes in cumulus-oocyte complexes

In vitro Maturation of Cattle Oocytes Taken from Ovaries with Different Morphofunctional State

Increased survival of preimplantation mouse embryos in medium with recombinant cytokine LIF

Influence of Combined Microinjection of Gene Engineering Construct and Endonuclease on Preimplantation Development of Mouse Embryos in vitro

Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro

Similar Documents

In vitro fertilization of bovine oocytes in protein-free culture system

Nonhormonal stimulation in vitro of oocyte maturation in sturgeons

Influence of culture medium osmolality on maturation and ovulation of common frog oocytes stimulated in vitro by pituitary extract or progesterone

Traditional and modern approaches to culture of preimplantation mammalian embryos in vitro

Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

Effects of NO-synthase inhibitors on maturation mouse oocytes in cumulus-oocyte complexes

In vitro Maturation of Cattle Oocytes Taken from Ovaries with Different Morphofunctional State

Increased survival of preimplantation mouse embryos in medium with recombinant cytokine LIF

Influence of Combined Microinjection of Gene Engineering Construct and Endonuclease on Preimplantation Development of Mouse Embryos in vitro

Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro