NDLI: Transmembrane transport of K$^{+}$ and Cl$^{−}$ during pollen grain activation in vivo and in vitro

Content Provider	Springer Nature Link
Author	Breygina, M. A. Matveyeva, N. P. Andreyuk, D. S. Yermakov, I. P.
Copyright Year	2012
Abstract	We studied the possibility of K$^{+}$ and Cl$^{−}$ efflux from tobacco pollen grains during their activation in vitro or on the stigma of a pistil. For this purpose the X-ray microanalysis and spectrofluorometry were applied. We found that the relative content of potassium and chlorine in the microvolume of pollen grain decreases during its hydration and activation on stigma. Efflux of these ions was found both in vivo and in vitro. In model in vitro experiments anion channel inhibitor NPPB ((5-nitro-2-(3-phenylpropylamino) benzoic acid) in the concentration that was blocking pollen germination, reduced Cl$^{−}$ efflux; potassium channel inhibitor TEA (tetraethylammonium chloride) partially reduced K$^{+}$ efflux and lowered the percent of activated cells. Another blocker of potassium channels Ba$^{2+}$ caused severe decrease in cell volume and blocked the activation. In general, the obtained data demonstrates that the initiation of pollen germination both in vivo and in vitro involves the activation of K$^{+}$ and Cl$^{−}$ release. An important role in these processes is played by NPPB-, TEA- and Ba$^{2+}$-sensitive plasmalemma ion channels.
Starting Page	85
Ending Page	93
Page Count	9
File Format	PDF
ISSN	10623604
Journal	Russian Journal of Developmental Biology
Volume Number	43
Issue Number	2
e-ISSN	16083326
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2012-03-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	male gametophyte pollen grain pollen germination on stigma chloride potassium X-ray microanalysis Developmental Biology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Developmental Biology

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