NDLI: Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions

Content Provider	Springer Nature Link
Author	Brodskii, V. Ya. Sharova, N. P. Mal’chenko, L. A. Konchenko, D. S. Dubovaya, T. K. Zvezdina, N. D.
Copyright Year	2015
Abstract	The effect of inhibition of proteasome activity on direct cell-cell interactions in primary hepatocyte cultures was studied. The circahoralian rhythm of protein synthesis was a marker of cell-cell communication. The addition of the proteasome inhibitor MG132 at doses of 10 or 20 μM to the medium with hepatocyte cultures for 19 h resulted in a significant reduction in the total pool of $^{3}$H-leucine in cells. The incorporation of leucine into proteins changed slightly or negligibly, whereas the content of free labeled leucine in hepatocytes decreased. The rhythm of protein synthesis was distorted compared to the control. The rhythm was restored by external organizers, such as gangliosides and melatonin, as well as by enhancing the activity of protein kinases—the key factor in the organization of the rhythm of protein synthesis. A short-term (3-h) exposure to MG132 did not change the pool of leucine, but the rhythm of protein synthesis was also disturbed. Thus, protein catabolism affects cell-cell interactions organizing the rhythm of protein synthesis. Another factor of the downregulation of the rhythm of protein synthesis, the secretion of proteins from the hepatocytes in vivo, which was shown in vivo in many studies, was also revealed in our study when measuring the content of proteins stained with Coomassie Brilliant Blue G250 in the medium with hepatocyte cultures.
Starting Page	38
Ending Page	45
Page Count	8
File Format	PDF
ISSN	10623604
Journal	Russian Journal of Developmental Biology
Volume Number	46
Issue Number	1
e-ISSN	16083326
Language	English
Publisher	Pleiades Publishing
Publisher Date	2015-01-21
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cell-cell interactions proteasomes inhibitor MG132 circahoralian rhythms rhythm of protein synthesis protein secretion hepatocytes Coomassie Brilliant Blue G250 Developmental Biology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Developmental Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes

Administration of Dopamine to Rats Disorganizes the Rhythm of Protein Synthesis in Hepatocytes

Mechanism of direct cell interactions. Self-organization of protein synthesis rhythm

Aftereffect of synchronizers of protein synthesis rhythm in hepatocytes in vitro

Age-related features of protein synthesis rhythm. Effects of extracellular medium

Melatonin promotes and synchronizes protein synthesis in hepatocyte culture from old rats

Interaction of Bioactive Coomassie Brilliant Blue G with Protein: Insights from Spectroscopic Methods

Signaling factors of self-organization of protein synthesis rhythm in hepatocyte cultures—gangliosides and catecholamines function independently

Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions

Similar Documents

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes

Administration of Dopamine to Rats Disorganizes the Rhythm of Protein Synthesis in Hepatocytes

Mechanism of direct cell interactions. Self-organization of protein synthesis rhythm

Aftereffect of synchronizers of protein synthesis rhythm in hepatocytes in vitro

Age-related features of protein synthesis rhythm. Effects of extracellular medium

Melatonin promotes and synchronizes protein synthesis in hepatocyte culture from old rats

Interaction of Bioactive Coomassie Brilliant Blue G with Protein: Insights from Spectroscopic Methods

Signaling factors of self-organization of protein synthesis rhythm in hepatocyte cultures—gangliosides and catecholamines function independently

Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions