NDLI: Protein S3 fragments neighboring mRNA during elongation and termination of translation on the human ribosome

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Protein S3 fragments neighboring mRNA during elongation and termination of translation on the human ribosome

Content Provider	Springer Nature Link
Author	Khairulina, Yu. S. Molotkov, M. V. Bulygin, K. N. Graifer, D. M. Ven’yamiva, A. G. Frolova, L. Yu. Stahl, J. Karpova, G. G.
Copyright Year	2008
Abstract	Protein S3 fragments were determined that crosslink to modified mRNA analogues in positions +5 to +12 relative to the first nucleotide in the P-site bound codon in model complexes mimicking states of ribosomes at the elongation and translation termination steps. The mRNA analogues contained a Phe codon UUU/UUC at the 5′-termini that could predetermine the position of the tRNA$^{Phe}$ on the ribosome by the P-site binding and perfluorophenylazidobenzoyl group at a nucleotide in various positions 3′ of the UUU/UUC codon. The crosslinked S3 protein was isolated from 80S ribosomal complexes irradiated with mild UV light and subjected to cyanogen bromide—induced cleavage at methionine residues with subsequent identification of the crosslinked oligopeptides. An analysis of the positions of modified oligopeptides resulting from the cleavage showed that, in dependence on the positions of modified nucleotides in the mRNA analogue, the crosslinking sites were found in the N-terminal half of the protein (fragment 2–217) and/or in the C-terminal fragment 190–236; the latter reflects a new peculiarity in the structure of the mRNA binding center in the ribosome, unknown to date. The results of crosslinking did not depend on the type of A-site codon or on the presence of translation termination factor eRF1.
Starting Page	691
Ending Page	697
Page Count	7
File Format	PDF
ISSN	10681620
Journal	Russian Journal of Bioorganic Chemistry
Volume Number	34
Issue Number	6
e-ISSN	1608330X
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2008-11-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ribosome photoaffinity cross-linking eukaryotes mRNA analogue mRNA binding site ribosomal protein S3 Biomedicine general Organic Chemistry Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry

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