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| Content Provider | Springer Nature Link |
|---|---|
| Author | Delgado, María C. Orsini Gallea, Mónica Añón, María C. Tironi, Valeria A. |
| Copyright Year | 2015 |
| Abstract | We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically relevant reactive species. The more active hydrolysate was obtained with the former method. Moreover, a prior alcalase treatment of the isolate followed by the same simulated gastrointestinal digestion did not improve the antioxidant capacity in any of the assays performed and even produced a negative effect under some conditions. Gastrointestinal digestion produced a strong increment in the scavenging capacity against peroxyl radicals (ORAC assay), hydroxyl radicals (ESR-OH assay), and peroxynitrites; thus decreasing the IC$_{50}$ values to approximately 20, 25, and 20 %, respectively, of the levels attained with the nonhydrolyzed proteins. Metal chelation (HORAC assay) also enhanced respect to isolate levels, but to a lesser extent (decreasing IC$_{50}$ values to only 50 %). The nitric-oxide– and superoxide-scavenging capacities of the digests were not relevant with respect to the methodologies used. The gastrointestinal digests from amaranth proteins acted against reactive species by different mechanisms, thus indicating the protein isolate to be a potential polyfunctional antioxidant ingredient. |
| Starting Page | 27 |
| Ending Page | 34 |
| Page Count | 8 |
| File Format | |
| ISSN | 09219668 |
| Journal | Plant Foods for Human Nutrition |
| Volume Number | 70 |
| Issue Number | 1 |
| e-ISSN | 15739104 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2015-01-11 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Amaranth proteins and peptides Gastrointestinal digestion Antioxidant activity Reactive oxygen and nitrogen species Food Science Chemistry/Food Science Nutrition Ecology Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Food Science |
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