NDLI: Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Content Provider	Springer Nature Link
Author	Uchiyama, Junji Kanesaki, Yu Iwata, Naoya Asakura, Ryousuke Funamizu, Kento Tasaki, Rizumu Agatsuma, Mina Tahara, Hiroko Matsuhashi, Ayumi Yoshikawa, Hirofumi Ogawa, Satoru Ohta, Hisataka
Copyright Year	2015
Abstract	Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-adapted Synechocystis sp. PCC 6803 (Synechocystis 6803) strains to identify genes involved in acid tolerance. Synechocystis 6803 is rarely found in habitants with pH < 5.75. The parent (P) strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our adapted cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-adapted and P strains, thereby identifying 11 SNPs in the acid-adapted strains, including in Fo F1-ATPase. To determine whether the SNP genes responded to acid stress, we examined gene expression in the adapted strains using quantitative reverse-transcription polymerase chain reaction. sll0914, sll1496, sll0528, and sll1144 expressions increased under acid stress in the P strain, whereas sll0162, sll0163, slr0623, and slr0529 expressions decreased. There were no differences in the SNP genes expression levels between the P strain and two adapted strains, except for sll0528. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803.
Starting Page	243
Ending Page	254
Page Count	12
File Format	PDF
ISSN	01668595
Journal	Photosynthesis Research
Volume Number	125
Issue Number	1-2
e-ISSN	15735079
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-03-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Low pH Stress response Synechocystis sp. PCC 6803 Experimental evolution Whole genome sequence Plant Sciences Plant Physiology Biochemistry Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine Biochemistry

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