NDLI: In vitro stereospecific hydration activities of the 3-vinyl group of chlorophyll derivatives by BchF and BchV enzymes involved in bacteriochlorophyll c biosynthesis of green sulfur bacteria

Content Provider	Springer Nature Link
Author	Teramura, Misato Harada, Jiro Tamiaki, Hitoshi
Copyright Year	2016
Abstract	The photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum produces bacteriochlorophyll (BChl) c pigments bearing a chiral 1-hydroxyethyl group at the 3-position, which self-aggregate to construct main light-harvesting antenna complexes, chlorosomes. The secondary alcoholic hydroxy group is requisite for chlorosomal aggregation and biosynthesized by hydrating the 3-vinyl group of their precursors. Using recombinant proteins of Cba. tepidum BchF and BchV, we examined in vitro enzymatic hydration of some 3-vinyl-chlorophyll derivatives. Both the enzymes catalyzed stereoselective hydration of zinc 3-vinyl-8-ethyl-12-methyl-bacteriopheophorbide c or d to the zinc 3$^{1}$ R-bacteriopheophorbide c or d homolog, respectively, with a slight amount of the 3$^{1}$ S-epimric species. A similar R-stereoselectivity was observed in the BchF-hydration of zinc 3-vinyl-8-ethyl- and propyl-12-ethyl-bacteriopheophorbides c, while their BchV-hydration gave a relatively larger amount of the 3$^{1}$ S-epimers. The in vitro stereoselective hydration confirmed the in vivo production of the S-epimeric species by BchV. The enzymatic hydration for the above 8-propylated substrate proceeded more slowly than that for the 8-ethylated, and the 8-isobutylated substrate was no longer hydrated. Based on these results, biosynthetic pathways of BChl c homologs and epimers are proposed.
Starting Page	33
Ending Page	45
Page Count	13
File Format	PDF
ISSN	01668595
Journal	Photosynthesis Research
Volume Number	130
Issue Number	1-3
e-ISSN	15735079
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-01-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chlorobaculum tepidum Chlorophyll Chlorosome Epimer Homolog Hydratase Plant Sciences Plant Physiology Biochemistry Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine Biochemistry

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