NDLI: Absorption spectra of chlorophyll a and b in Lhcb protein environment

Content Provider	Springer Nature Link
Author	Cinque, Gianfelice Croce, Roberta Bassi, Roberto
Copyright Year	2000
Abstract	The spectral forms of the two chlorophyll species in higher plant Photosystem II antenna proteins have been experimentally determined within their protein environment. Recombinant CP29 and LHC II antenna proteins missing individual chromophores were obtained by over-expression in bacteria without any changing of the primary protein sequence and in vitro reconstitution. Difference absorption spectroscopy with respect to the corresponding proteins binding the complete pigment complement yielded the spectral shape and extinction of single chlorophyll a and b. A functional relation of their absorption was given by Gaussian subband decomposition covering the entire Q$_{x}$ and Q$_{y}$ optical region together with the absolute value of the molar extinction coefficient. With respect to analogous determinations reported in the literature for organic solvents, this information is valuable for further understanding the in-protein chlorophyll excited states and excited state dynamics: in particular, for the calculation of Förster transfer rates by means of chlorophyll–chlorophyll overlap integral employing the Stepanov relation for emission and single chromophore transition energies according to the results of mutational analysis of chlorophyll binding sites [Bassi et al. (1999) Proc Natl Acad Sci USA 96: 10056–10061; Remelli et al. (1999) J Biol Chem 274: 33510–33521].
Starting Page	233
Ending Page	242
Page Count	10
File Format	PDF
ISSN	01668595
Journal	Photosynthesis Research
Volume Number	64
Issue Number	2-3
e-ISSN	15735079
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biosynthesis of chlorophyll b and the chlorophyll cycle

Oceanic picophytoplankton having a high abundance of chlorophyll b in the major light harvesting chlorophyll protein complex

Analysis of the chlorophyll biosynthetic system in a chlorophyll b-less barley mutant

Chlorophyllide a Oxygenase mRNA and Protein Levels Correlate with the Chlorophyll a/b Ratio in Arabidopsis thaliana

Physical separation of chlorophyll-protein complexes

Evidence for an O$_{2}$-barrier in the light-harvesting chlorophyll-a/b-protein complex LHC II

Spectrofluorimetric method for the determination of large chlorophyll a/b ratios

New multichannel kinetic spectrophotometer–fluorimeter with pulsed measuring beam for photosynthesis research

Chlorophyll b degradation by chlorophyll b reductase under high-light conditions

Absorption spectra of chlorophyll a and b in Lhcb protein environment

Similar Documents

Biosynthesis of chlorophyll b and the chlorophyll cycle

Oceanic picophytoplankton having a high abundance of chlorophyll b in the major light harvesting chlorophyll protein complex

Analysis of the chlorophyll biosynthetic system in a chlorophyll b-less barley mutant

Chlorophyllide a Oxygenase mRNA and Protein Levels Correlate with the Chlorophyll a/b Ratio in Arabidopsis thaliana

Physical separation of chlorophyll-protein complexes

Evidence for an O$_{2}$-barrier in the light-harvesting chlorophyll-a/b-protein complex LHC II

Spectrofluorimetric method for the determination of large chlorophyll a/b ratios

New multichannel kinetic spectrophotometer–fluorimeter with pulsed measuring beam for photosynthesis research

Chlorophyll b degradation by chlorophyll b reductase under high-light conditions

Absorption spectra of chlorophyll a and b in Lhcb protein environment