NDLI: A novel and mild isolation procedure of chlorosomes from the green sulfur bacterium Chlorobaculum tepidum

Content Provider	Springer Nature Link
Author	Tokita, Seiji Shimada, Keizo Watabe, Kazuyuki Matsuura, Katsumi Mimuro, Mamoru
Copyright Year	2011
Abstract	In this article, we developed a new and mild procedure for the isolation of chlorosomes from a green sulfur bacterium Chlorobaculum tepidum. In this procedure, Fenna–Matthews–Olson (FMO) protein was released by long cold treatment (6°C) of cells under the presence of a chaotrope (2 M NaSCN) and 0.6 M sucrose. Chlorosomes were released by an osmotic shock of the cold-treated cells after the formation of spheroplasts without mechanical disruption. Chlorosomes were finally purified by a sucrose step-wise density gradient centrifugation. We obtained two samples with different density (20 and 23% sucrose band, respectively) and compared them by SDS-PAGE, absorption spectroscopy at 80 K, fluorescence and CD spectroscopy at room temperature. Cells whose absorption maximum was longer than 750 nm yielded higher amount of the 20% sucrose fraction than those having an absorption maximum shorter than 750 nm.
Starting Page	183
Ending Page	190
Page Count	8
File Format	PDF
ISSN	01668595
Journal	Photosynthesis Research
Volume Number	108
Issue Number	2-3
e-ISSN	15735079
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-08-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Isolation method Chlorosomes FMO protein Low-temperature absorption Chlorobaculum tepidum Plant Genetics & Genomics Biochemistry Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine Biochemistry

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