NDLI: A Fluorescence Detected Magnetic Resonance Investigation of the Carotenoid Triplet States Associated with Photosystem II of Isolated Spinach Thylakoid Membranes

Content Provider	Springer Nature Link
Author	Santabarbara, Stefa Agostini, Giancarlo Heathcote, Peter Carbonera, Donatella
Copyright Year	2005
Abstract	The carotenoid triplet populations associated with the fluorescence emission chlorophyll forms of Photosystem II have been investigated in isolated spinach thylakoid membranes by means of fluorescence detected magnetic resonance in zero field (FDMR). The spectra collected in the 680–690 nm emission range, have been fitted by a global analysis procedure. At least five different carotenoid triplet states coupled to the terminal emitting chlorophyll forms of PS II, peaking at 682 nm, 687 nm and 692 nm, have been characterised. The triplets associated with the outer antenna emission forms, at 682 nm, have zero field splitting parameters \|D\| = 0.0385 cm$^{−1}$, \|E\| = 0.00367 cm$^{−1}$; \|D\| = 0.0404 cm$^{−1}$, \|E\| = 0.00379 cm$^{−1}$ and \|D\| = 0.0386 cm$^{−1}$, \|E\| = 0.00406 cm$^{−1}$ which are very similar to those previously reported for the xanthophylls of the isolated LHC II complex. Therefore the FDMR spectra recorded in this work provide insights into the organisation of the LHC II complex in the unperturbed environment represented by thylakoid membranes. The additional carotenoid triplet populations, detected by monitoring the chlorophyll emission at 687 and 692 nm, are assigned to carotenoids bound to inner antenna complexes and hence attributed to β-carotene molecules.
Starting Page	283
Ending Page	296
Page Count	14
File Format	PDF
ISSN	01668595
Journal	Photosynthesis Research
Volume Number	86
Issue Number	1-2
e-ISSN	15735079
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	carotenoid light harvesting complexes ODMR Photosystem II triplet states Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine Biochemistry

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