NDLI: Overexpression of a Malus xiaojinensis Nas1 Gene Influences Flower Development and Tolerance to Iron Stress in Transgenic Tobacco

Content Provider	Springer Nature Link
Author	Han, De Guo Yang, Guo Hui Xu, Ke Dong Shao, Qin Yu, Ze Yuan Wang, Bing Ge, Qiu Lai Yu, Yang
Copyright Year	2013
Abstract	Iron (Fe) is one of the essential micronutrients required by all living organisms. Expression of MxNas1 of Malus xiaojinensis is enriched in active tissues, including leaves, roots, and phloem, and these seedlings are highly affected by Fe stress and indoleacetic acid treatment, but weakly affected by abscisic acid treatment. Subcellular localization has revealed that MxNas1 is preferentially localized in cell membranes. When this gene is introduced into tobacco, it promotes synthesis of nicotianamine (NA) synthase and increases NA content. Overexpression of MxNas1 improves tolerance to Fe stress in transgenic tobacco, but also leads to misshapen flowers. Higher levels of MxNas1 expression in transgenic tobacco plants also contributes to delayed flowering and increased levels of Fe and Zn in flowers. In addition to its role in metal transport in plants, NA may be involved in the regulation of metal transfer within cells. These results suggest that NA excess influences functions of metal-requiring proteins, including some of the transcription factors.
Starting Page	802
Ending Page	809
Page Count	8
File Format	PDF
ISSN	07359640
Journal	Plant Molecular Biology Reporter
Volume Number	31
Issue Number	4
e-ISSN	15729818
Language	English
Publisher	Springer US
Publisher Date	2013-01-10
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Iron MxNas1 Strategy I Transgenic tobacco Real-time PCR Plant Sciences Plant Breeding/Biotechnology Proteomics Metabolomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Plant Science Molecular Biology

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