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| Content Provider | Springer Nature Link |
|---|---|
| Author | Koizumi, Mayuko Shimotori, Yumi Saeki, Yuta Hirai, Sayaka Oka, Shin ichiro Kodama, Hiroaki |
| Copyright Year | 2017 |
| Abstract | The Cucumber mosaic virus (CMV) 2b protein has multiple activities as a viral suppressor of RNA silencing (VSR). Here, we characterized the 2b protein (IA2b) of a CMV-IA isolate. This IA2b protein has two nuclear localization signals, and when it was fused with GFP, the protein was found to be preferentially localized in the nucleus. A transgenic tobacco line, 2b8, expressing the IA2b gene showed an enhanced expression of an agroinfiltrated sense transgene, which indicated that IA2b has VSR activity. When 2b8 plants were crossed with plants showing sense-transgene-induced post-transcriptional gene silencing (S-PTGS), the IA2b protein caused the disappearance of small interfering RNAs (siRNAs) and the S-PTGS phenotype was converted to the overexpression phenotype. When 2b8 plants were crossed with plants showing inverted repeat-induced post-transcriptional gene silencing (IR-PTGS), the IA2b protein did not suppress the accumulation of siRNAs processed from hairpin RNAs and the silencing phenotype was maintained. When a tobacco plant showing an overexpressed phenotype of a sense transgene was crossed with IR-PTGS plants targeting the same transgene, the primary siRNAs that were produced by the IR-PTGS construct triggered the production of the secondary siRNAs through the RNA-dependent RNA polymerase6 (RDR6)-dependent pathway. The IA2b protein suppressed the secondary siRNA formation but not the primary siRNA production from hairpin RNAs, resulting in the silencing phenotype from IR-PTGS being maintained. These results indicate that IA2b suppresses RDR6-dependent siRNA production and does not interfere with the silencing step by siRNA-guided RNA degradation. |
| Starting Page | 265 |
| Ending Page | 272 |
| Page Count | 8 |
| File Format | |
| ISSN | 07359640 |
| Journal | Plant Molecular Biology Reporter |
| Volume Number | 35 |
| Issue Number | 2 |
| e-ISSN | 15729818 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2017-01-05 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | 2b protein Agroinfiltration Cucumber mosaic virus IR-PTGS S-PTGS Transitivity Plant Sciences Plant Breeding/Biotechnology Proteomics Metabolomics Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Molecular Biology |
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