NDLI: Carbon cost of nitrogenase activity in Frankia

Content Provider	Springer Nature Link
Author	Lundquist, P. O.
Copyright Year	2005
Abstract	The carbon cost of nitrogenase activity was investigated to determine symbiotic efficiency of the actinorhizal root nodule symbiosis between the woody perennial Alnus incana and the soil bacterium Frankia. Respiration (CO$_{2}$ production) and nitrogenase activity (H$_{2}$ production) by intact nodulated root systems were continuously recorded in short-term assays in an open-flow gas exchange system. The assays were conducted in N$_{2}$:O$_{2}$, thus under N$_{2}$-fixing conditions, in all experiments except for one. This avoided the declines in nitrogenase activity and respiration due to N$_{2}$ deprivation that occur in acetylene reduction assays and during extended Ar:O$_{2}$ exposures in H$_{2}$ assays. Two approaches were used: (i) direct estimation of root and nodule respiration by removing nodules, and (ii) decreasing the partial pressure of O$_{2}$ from 21 to 15% to use the strong relationship between respiration and nitrogenase activity to calculate CO$_{2}$/H$_{2}$. The electron allocation of nitrogenase was determined to be 0.6 and used to convert the results into moles of CO$_{2}$ produced per 2e$^{−}$ transferred by nitrogenase to reduction of N$_{2}$. The results ranged from 2.6 to 3.4 mol CO$_{2}$ produced per 2e$^{−}$. Carbon cost expressed as g C produced per g N reduced ranged from 4.5 to 5.8. The result for this actinorhizal tree symbiosis is in the low range of estimates for N$_{2}$-fixing actinorhizal symbioses and crop legumes. Methodology and comparisons of root nodule physiology among actinorhizal and legume plants are discussed.
Starting Page	235
Ending Page	244
Page Count	10
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	273
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	actinorhiza Alnus incana Frankia N$_{2}$ fixation respiration symbiosis Ecology Plant Sciences Plant Physiology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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