NDLI: Proteome analysis of soybean roots subjected to short-term drought stress

Content Provider	Springer Nature Link
Author	Alam, Iftekhar Sharmin, Shamima Akhtar Kim, Kyung Hee Yang, Jae Kyung Choi, Myung Suk Lee, Byung Hyun
Copyright Year	2010
Abstract	Drought is one of the most important constraints on the growth and productivity of many crops, including soybeans. However, as a primary sensing organ, the plant root response to drought has not been well documented at the proteomic level. In the present study, we carried out a proteome analysis in combination with physiological analyses of soybean roots subjected to severe but recoverable drought stress at the seedling stage. Drought stress resulted in the increased accumulation of reactive oxygen species and subsequent lipid peroxidation. The proline content increased in drought-stressed plants and then decreased during the period of recovery. The high-resolution proteome map demonstrated significant variations in about 45 protein spots detected on Comassie briliant blue-stained 2-DE gels. Of these, 28 proteins were identified by mass spectrometry; the levels of 5 protein spots were increased, 21 were decreased and 2 spots were newly detected under drought condition. When the stress was terminated by watering the plants for 4 days, in most cases, the protein levels tended towards the control level. The proteins identified in this study are involved in a variety of cellular functions, including carbohydrate and nitrogen metabolism, cell wall modification, signal transduction, cell defense and programmed cell death, and they contribute to the molecular mechanism of drought tolerance in soybean plants. Analysis of protein expression patterns revealed that proteins associated with osmotic adjustment, defense signaling and programmed cell death play important roles for soybean plant drought adaptation. The identification of these proteins provides new insight that may lead to a better understanding of the molecular basis of the drought stress responses.
Starting Page	491
Ending Page	505
Page Count	15
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	333
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-04-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress Drought Programmed cell death Proteome Soybean Ecology Plant Physiology Soil Science & Conservation Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean

Proteome analysis of soybean roots under waterlogging stress at an early vegetative stage

Short-term salinity stress in tobacco plants leads to the onset of animal-like PCD hallmarks in planta in contrast to long-term stress

Nitrate uptake ability by maize roots during and after drought stress

Cell death in wheat roots induced by the powdery mildew fungus Blumeria graminis f. sp. tritici

Oxidative stress is associated with aluminum toxicity recovery in apex of pea root

Hydrogen peroxide generation and antioxidant enzyme activities in the leaves and roots of wheat cultivars subjected to long-term soil drought stress

Dynamic root responses to drought and rewatering in two wheat (Triticum aestivum) genotypes

Drought priming at vegetative stage improves the antioxidant capacity and photosynthesis performance of wheat exposed to a short-term low temperature stress at jointing stage

Proteome analysis of soybean roots subjected to short-term drought stress

Similar Documents

Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean

Proteome analysis of soybean roots under waterlogging stress at an early vegetative stage

Short-term salinity stress in tobacco plants leads to the onset of animal-like PCD hallmarks in planta in contrast to long-term stress

Nitrate uptake ability by maize roots during and after drought stress

Cell death in wheat roots induced by the powdery mildew fungus Blumeria graminis f. sp. tritici

Oxidative stress is associated with aluminum toxicity recovery in apex of pea root

Hydrogen peroxide generation and antioxidant enzyme activities in the leaves and roots of wheat cultivars subjected to long-term soil drought stress

Dynamic root responses to drought and rewatering in two wheat (Triticum aestivum) genotypes

Drought priming at vegetative stage improves the antioxidant capacity and photosynthesis performance of wheat exposed to a short-term low temperature stress at jointing stage

Proteome analysis of soybean roots subjected to short-term drought stress