NDLI: Influence of inoculum density of the antagonistic bacteria Pseudomonas fluorescens and Pseudomonas corrugata on sugar beet seedling colonisation and suppression of Pythium damping off

Content Provider	Springer Nature Link
Author	Schmidt, C. S. Agostini, F. Leifert, C. Killham, K. Mullins, C. E.
Copyright Year	2004
Abstract	The effect of initial inoculum density of the antagonistic bacterial strains Pseudomonas fluorescens B5 and Pseudomonas corrugata 2140 (10$^{3}$ to 10$^{8}$ CFU per seed pellet) on sugar beet seedling colonisation, in situ bioluminescence and antagonistic activity towards Pythium ultimum was investigated. Populations of the bacteria colonising sugar beet root systems approached an apparent carrying capacity of 10$^{5}$ to 10$^{6}$ CFU per plant after 12 d growth, irrespective of inoculum density. This meant an up to 320-fold population increase at low inoculum densities and a decrease at high densities. Population densities of both bacteria and their corresponding in situ bioluminescence (resulting from luciferase enzyme expression from the inserted luxAB genes) reached highest levels in the hypocotyl region and in the upper root region 0–20 mm below seed level (10$^{4}$–10$^{6}$ CFU/cm section, 10$^{1}$–10$^{3}$ RLU/cm section) and decreased with root depth. In situ bioluminescence, which indicates physiological activity, was measurable at lowest antagonist initial inoculum density (10$^{3}$ CFU per seed pellet) and did not increase significantly with increasing inoculum density. Bioluminescence was also significantly correlated with population density. For Pseudomonas fluorescens B5, the total population size per plant and downward colonisation of the root (below 40 mm depth) increased significantly with antagonist inoculum density applied to the seeds. For Pseudomonas corrugata 2140, no significant influence of initial inoculum density on root colonisation was observable. Survival and dry weight of sugar beet seedlings in Pythium infested soil increased significantly with increasing inoculum density of Pseudomonas fluorescens B5, whereas for Pseudomonas corrugata 2140, initial densities of 10$^{4}$ to 10$^{6}$ CFU per seed resulted in maximal survival of plants.
Starting Page	111
Ending Page	122
Page Count	12
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	265
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	antagonism bioluminescence dose-response lux-marking rhizosphere root colonization Ecology Plant Sciences Plant Physiology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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