NDLI: 31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi

Content Provider	Springer Nature Link
Author	Rasmussen, Nanna Lloyd, David C. Ratcliffe, R. George Hansen, Poul Erik Jakobsen, Iver
Copyright Year	2000
Abstract	$^{31}$P nuclear magnetic resonance (NMR) spectroscopy was used to study phosphate (P) metabolism in mycorrhizal and nonmycorrhizal roots of cucumber (Cucumis sativus L) and in external mycelium of the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck & Smith. The in vivo NMR method allows biological systems to be studied non-invasively and non-destructively. $^{31}$P NMR experiments provide information about cytoplasmic and vacuolar pH, based on the pH-dependent chemical shifts of the signals arising from the inorganic P (P$_{i}$) located in the two compartments. Similarly, the resonances arising from α, β and γ phosphates of nucleoside triphosphates (NTP) and nucleoside diphosphates (NDP) supply knowledge about the metabolic activity and the energetic status of the tissue. In addition, the kinetic behaviour of P uptake and storage can be determined with this method. The $^{31}$P NMR spectra of excised AM fungi and mycorrhizal roots contained signals from polyphosphate (PolyP), which were absent in the spectra of nonmycorrhizal roots. This demonstrated that the P$_{i}$ taken up by the fungus was transformed into PolyP with a short chain length. The spectra of excised AM fungi revealed only a small signal from the cytoplasmic P$_{i}$, suggesting a low cytoplasmic volume in this AM fungus.
Starting Page	245
Ending Page	253
Page Count	9
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	226
Issue Number	2
e-ISSN	15735036
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecology Plant Sciences Plant Physiology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi. Metabolism and transport in AM fungi

Arbuscular mycorrhizal fungi in two mangroves in South China

Putative sites for nutrient uptake in arbuscular mycorrhizal fungi

Molecular identification and phylogeny of arbuscular mycorrhizal fungi

The relationship between the diversity of arbuscular mycorrhizal fungi and grazing in a meadow steppe

Phosphate transporters of Medicago truncatula and arbuscular mycorrhizal fungi

Associations between arbuscular mycorrhizal fungi and Rhynchrelyrum repens in abandoned quarries in southern China

Wheat Responses to Arbuscular Mycorrhizal Fungi in a Highly Calcareous Soil Differ from those of Clover, and Change with Plant Development and P supply

Is there a role for arbuscular mycorrhizal fungi in production agriculture?

31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi

Similar Documents

Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi. Metabolism and transport in AM fungi

Arbuscular mycorrhizal fungi in two mangroves in South China

Putative sites for nutrient uptake in arbuscular mycorrhizal fungi

Molecular identification and phylogeny of arbuscular mycorrhizal fungi

The relationship between the diversity of arbuscular mycorrhizal fungi and grazing in a meadow steppe

Phosphate transporters of Medicago truncatula and arbuscular mycorrhizal fungi

Associations between arbuscular mycorrhizal fungi and Rhynchrelyrum repens in abandoned quarries in southern China

Wheat Responses to Arbuscular Mycorrhizal Fungi in a Highly Calcareous Soil Differ from those of Clover, and Change with Plant Development and P supply

Is there a role for arbuscular mycorrhizal fungi in production agriculture?

31P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi