NDLI: Bicarbonate as tracer for assimilated C and homogeneity of $^{14}$C and $^{15}$N distribution in plants by alternative labeling approaches

Content Provider	Springer Nature Link
Author	Rasmussen, Jim Kusliene, Gedrime Jacobsen, Ole Stig Kuzyakov, Yakov Eriksen, Jørgen
Copyright Year	2013
Abstract	Application of carbon (C) and nitrogen (N) isotopes is an essential tool to study C and N flows in plant-soil-microorganisms systems. When targeting single plants in a community the tracers need to be added via e.g., leaf-labeling or stem-feeding approaches. In this study we: (i) investigated if bicarbonate can be used to introduce $^{14}$C (or $^{13}$C) into white clover and ryegrass, and (ii) compared the patterns of $^{14}$C and $^{15}$N allocation in white clover and ryegrass to evaluate the homogeneity of tracer distribution after two alternative labeling approaches.Perennial ryegrass and white clover were pulse labeled with $^{15}$N urea via leaf-labeling and $^{14}$C either via a $^{14}$CO$_{2}$ atm or with $^{14}$C bicarbonate through leaf-labeling. Plants were sampled 4 days after labeling and prepared for bulk isotope analysis and for $^{14}$C imaging to identify plant parts with high and low $^{14}$C activity. Subsequently, plant parts with high and low $^{14}$C activity were separated and analyzed for $^{15}$N enrichment.Bicarbonate applied by leaf-labeling efficiently introduced $^{14}$C into both white clover and ryegrass, although the $^{14}$C activity in particular for white clover was found predominantly in the labeled leaf. Using $^{14}$C imaging for identification of areas with high (hotspots) and low $^{14}$C activity showed that $^{14}$C was incorporated very heterogeneously both when using bicarbonate and CO$_{2}$ as expected when using pulse labeling. Subsequent analysis of $^{15}$N enrichment in plant parts with high and low $^{14}$C activity showed that $^{15}$N also had a heterogeneous distribution (up to two orders of magnitude).Bicarbonate can efficiently be used to introduce $^{14}$C or $^{13}$C into plant via the leaf-labeling method. Both $^{14}$C and $^{15}$N showed heterogeneous distribution in the plant, although the distribution of $^{15}$N was more even than that of $^{14}$C.
Starting Page	191
Ending Page	198
Page Count	8
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	371
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Allocation of assimilates Tracer allocation $^{15}$N distribution N utilization $^{14}$C imaging Isotopic applications Pulse labeling Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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