NDLI: A randomization method for efficiently and accurately processing fine roots, and separating them from debris, in the laboratory

Content Provider	Springer Nature Link
Author	Koteen, Laura E. Baldocchi, Dennis D.
Copyright Year	2012
Abstract	We developed a method for processing roots from soil cores and monoliths in the laboratory to reduce the time and cost devoted to separating roots from debris and improve the accuracy of root variable estimates. The method was tested on soil cores from a California oak savanna, with roots from trees, Quercus douglasii, and annual grasses.In the randomized sampling method, one isolates the sample fraction consisting of roots and organic debris < = 1 cm in length, and randomizes it through immersion in water and vigorous mixing. Sub-samples from the mixture are then used to estimate the percentage of roots in this fraction, thereby enabling an estimate of total sample biomass.We found that root biomass estimates, determined through the randomization method, differed from total root biomass established by meticulously picking every root from a sample with an error of 3.0 % +/− 0.6 % s.e.This method greatly reduces the time and resources required for root processing from soil cores and monoliths, and improves the accuracy of root variable estimates compared to standard methods. This gives researchers the ability to increase sample frequency and reduce the error associated with studying roots at the landscape and plant scales.
Starting Page	383
Ending Page	398
Page Count	16
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	363
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-08-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Quercus douglasii Population sampling Randomization analysis Root processing methods Root separation from debris Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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Biomass and distribution of fine and coarse roots from blue oak (Quercus douglasii) trees in the northern Sierra Nevada foothills of California

Contrasting seasonal patterns of fine root production for blue oaks (Quercus douglasii) and annual grasses in California oak woodland

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A randomization method for efficiently and accurately processing fine roots, and separating them from debris, in the laboratory

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