NDLI: Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H$^{+}$ ATPase in rice roots

Content Provider	Springer Nature Link
Author	Zeng, Houqing Liu, Gan Kishita, Toshiri Zhang, Ruiping Zhu, Yiyong Shen, Qirong Xu, Guohua
Copyright Year	2012
Abstract	Nitrogen, especially NH 4 + , can stimulate the uptake of phosphorus in plants, but the underlying mechanisms have not been clearly elucidated. Because phosphate is taken up via an anion/H$^{+}$ co-transport process, we propose that the stimulated uptake of phosphorus by NH 4 + versus NO 3 - nutrition may be related to the activity of plasma membrane H$^{+}$ ATPase. In the present study, we investigated the effect of NH 4 + and NO 3 - on phosphorus uptake and plasma membrane H$^{+}$ ATPase activity in rice.Rice plants were cultivated in a hydroponic solution with NH 4 + or NO 3 - . After 15 days of cultivation, phosphorus content was determined. Root plasma membrane was isolated using a two-phase partitioning system and hydrolytic H$^{+}$-ATPase activity was determined by measuring the P$_{i}$ concentration after a 30-min hydrolysis reaction. Relative expression of plasma membrane H$^{+}$ ATPase genes was analyzed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). H$^{+}$ ATPase enzyme concentration in the plasma membrane was detected by western blot. For $^{33}$P uptake experiments, rice roots were incubated in the nutrient solution with addition of H 3 33 PO$_{4}$.P content in both the roots and shoots of rice plants supplemented with NH 4 + was significantly higher than P content in plants grown with NO 3 - . Plasma membrane H$^{+}$ ATPase activity in NH 4 + -fed rice roots was significantly higher than that in NO 3 - -fed rice roots. Real-time qRT-PCR and western blot results indicated that the higher activity of plasma membrane H$^{+}$ ATPase in NH 4 + -fed rice roots could be attributed to increased expression of the OSA1, OSA3, OSA7, OSA8 and OSA9 genes and an increase in H$^{+}$ ATPase enzyme concentration in the plasma membrane. Results from $^{33}$P uptake experiments showed that rice roots incubated with NH 4 + absorbed more $^{33}$P during the four-hour incubation than did rice roots incubated with NO 3 - . Vanadate inhibited $^{33}$P uptake in rice roots supplied with NH 4 + , while fusicoccin stimulated $^{33}$P uptake under NO 3 - nutrition.Taken together, these results suggest an involvement of plasma membrane H$^{+}$ ATPase in the stimulated uptake of phosphorus by rice roots supplemented with NH 4 + .
Starting Page	205
Ending Page	214
Page Count	10
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	357
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ammonium Nitrate Phosphorus uptake Plasma membrane H$^{+}$ ATPase Rice (Oryza sativa L.) Plant Sciences Plant Physiology Ecology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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