NDLI: Transfer RNA-dependent asparagine biosynthesis in Gluconacetobacter diazotrophicus and its influence on biological nitrogen fixation

Content Provider	Springer Nature Link
Author	Alquéres, Sylvia M. Cardoso, Alexander M. Brito Moreira, Jorda Baldani, Jose I. Martins, Orlando B.
Copyright Year	2011
Abstract	Gluconacetobacter diazotrophicus is a nitrogen-fixing endophytic bacterium isolated from sugarcane, rice, elephant grass, sweet potato, coffee, and pineapple. These plants have high level of asparagine, which promotes microbial growth and inhibits nitrogenase activity. The regulation of intracellular concentrations of this amino acid is essential for growth and biological nitrogen fixation (BNF) in this diazotroph; however its asparagine metabolic pathway has not yet been clearly established.The work reported here is the first to demonstrate the use of an alternative route for asparaginyl-tRNA (Asn-tRNA) and asparagine formation in an endophytic nitrogen-fixing bacterium by using in silico and in vitro analysis.The indirect route involves transamidation of incorrectly charged tRNA via GatCAB transamidase. Nitrogenase activity was completely inhibited by 20 mM Asn in LGI-P medium, which in contrast promotes protein synthesis and microbial growth.The analysis carried out in this work shows that intracellular levels of asparagine regulate the expression of nitrogenase nifD gene (GDI0437), suggesting that the presence of an alternative route to produce asparagine might give the G. diazotrophicus a tighter control over cell growth and BNF, and may be of importance in the regulation of the endophytic plant-microbe interaction.
Starting Page	209
Ending Page	216
Page Count	8
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	356
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-08-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gluconacetobacter diazotrophicus tRNA Asparagine biosynthesis Biological nitrogen fixation Ecology Soil Science & Conservation Plant Sciences Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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