NDLI: A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.)

Content Provider	Springer Nature Link
Author	Arkoun, Mustapha Jannin, Laëtitia Laîné, Philippe Etienne, Philippe Masclaux Daubresse, Céline Citerne, Sylvie Garnica, Maria Garcia Mina, José Maria Yvin, Jean Claude Ourry, Alain
Copyright Year	2012
Abstract	Urea is the major nitrogen (N) form supplied as fertilizer in agriculture. However, urease, a nickel-dependent enzyme, allows plants to use external or internally generated urea as a nitrogen source. Since a urease inhibitor is frequently applied in conjunction with urea fertilizer, the N-metabolism of plants may be affected. The aim of this study was to determine physiological and molecular effects of nickel deficiency and a urease inhibitor on urea uptake and assimilation in oilseed rape.Plants were grown on hydroponic solution with urea as the sole N source under three treatments: plants treated with nickel (+Ni) as a control, without nickel (−Ni) and with nickel and phenylphosphorodiamidate (+Ni+PPD). Urea transport and assimilation were investigated.The results show that Ni-deficiency or PPD supply led to reduced growth and reduced $^{15}$N-uptake from urea. This effect was more pronounced in PPD-treated plants, which accumulated high amounts of urea and ammonium. Thus, Ni-deficiency or addition of PPD, limit the availability of N and decreased shoot and root amino acid content. The up-regulation of BnDUR3 in roots indicated that this gene is a component of the stress response to nitrogen-deficiency. A general decline of glutamine synthetase (GS) activity and activation of glutamate dehydrogenase (GDH) and increases in its expression level were observed in control plants. At the same time, in (−N) or (+Ni+PPD) treated plants, no increases in GS or GDH activities and expression level were found.Overall results showed that plants require Ni as a nutrient (while most widely used nutrient solutions are devoid of Ni), whether they are grown with or without a urea supply, and that urease inhibitors may have deleterious effects at least in hydroponic grown oilseed rape.
Starting Page	79
Ending Page	92
Page Count	14
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	362
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-05-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Urea BnDUR3 $^{15}$N-labeling Nickel Phenylphosphorodiamidate (PPD) Enzymatic activities Gene expressions Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Turnover of residual $^{15}$N-labelled fertilizer N in soil following harvest of oilseed rape (t Brassica napus L.)

Diagnosing sulfur deficiency in field-grown oilseed rape (Brassica napus L.) and wheat ( Triticum aestivum L.)

Genome-scale mRNA transcriptomic insights into the responses of oilseed rape (Brassica napus L.) to varying boron availabilities

The role of boron nutrition in seed vigour of oilseed rape (Brassica napus L.)

Differential response of oilseed rape (Brassica napus L.) cultivars to low boron supply

Boron efficiency in oilseed rape: I. Genotypic variation demonstrated in field and pot grown Brassica napus L. and Brassica juncea L.

Plant regeneration from hypocotyl protoplasts of winter oilseed rape (Brassica napus L.)

Regulatory effects of sulfur on oilseed rape (Brassica napusL.) response to selenite

Zinc efficiency of oilseed rape (t Brassica napus and t B. juncea) genotypes

A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.)

Similar Documents

Turnover of residual $^{15}$N-labelled fertilizer N in soil following harvest of oilseed rape (t Brassica napus L.)

Diagnosing sulfur deficiency in field-grown oilseed rape (Brassica napus L.) and wheat ( Triticum aestivum L.)

Genome-scale mRNA transcriptomic insights into the responses of oilseed rape (Brassica napus L.) to varying boron availabilities

The role of boron nutrition in seed vigour of oilseed rape (Brassica napus L.)

Differential response of oilseed rape (Brassica napus L.) cultivars to low boron supply

Boron efficiency in oilseed rape: I. Genotypic variation demonstrated in field and pot grown Brassica napus L. and Brassica juncea L.

Plant regeneration from hypocotyl protoplasts of winter oilseed rape (Brassica napus L.)

Regulatory effects of sulfur on oilseed rape (Brassica napusL.) response to selenite

Zinc efficiency of oilseed rape (t Brassica napus and t B. juncea) genotypes

A physiological and molecular study of the effects of nickel deficiency and phenylphosphorodiamidate (PPD) application on urea metabolism in oilseed rape (Brassica napus L.)