NDLI: Direct measurement of roots in soil for single and mixed species using a quantitative DNA-based method

Content Provider	Springer Nature Link
Author	Haling, Rebecca E. Simpson, Richard J. McKay, Alan C. Hartley, Diana Lambers, Hans Ophel Keller, Kathy Wiebkin, Sue Riley, Ian T. Richardson, Alan E.
Copyright Year	2011
Abstract	Molecular techniques present a new opportunity to study roots and their interactions in soil. Extraction and quantification of species-specific DNA directly from soil allows direct identification of roots in mixed swards reducing the need for labour-intensive methods to recover and identify individual roots. DNA was extracted directly from up to 0.5 kg of soil and the presence of individual species quantified using species-specific probes with quantitative real-time PCR. A range of plant and soil factors influenced the DNA content measured in roots and it was necessary to account for these influences when converting DNA amount to root mass. The utility of the method for quantitative root studies was demonstrated in an experiment to investigate the effect of lime on root growth of acid-soil resistant and sensitive perennial grasses grown together in an aluminium-toxic soil. The root mass of an acid-soil resistant species was unaffected by lime application, whereas that of an acid-soil sensitive species was restricted by soil acidity. Molecular techniques present a promising tool for quantification of root mass directly in soil and have applications for field studies involving mixed species of plants.
Starting Page	123
Ending Page	137
Page Count	15
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	348
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phalaris Weeping grass Tall wheatgrass Lucerne Alfalfa Subterranean clover Phalaris aquatica Microlaena stipoides Thinopyrum ponticum Medicago sativa Trifolium subterraneum pH Plant Physiology Soil Science & Conservation Plant Sciences Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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