NDLI: Zinc supply impacts on the relative expression of a metallothionein-like gene in Coffea arabica plants

Content Provider	Springer Nature Link
Author	Barbosa, Bárbara Castanheira Ferrara Silva, Samuel Chaves Oliveira, Raphael Ricon Chalfun, Antonio
Copyright Year	2016
Abstract	Zinc (Zn) is an essential nutrient for plants, as it functions as a component and cofactor of enzymes. Metallothioneins (MTs), are known to participate in the process of metal homeostasis have been hypothesized to be partially responsible for the distribution of Zn in plants. We aimed to characterize putative MT-like genes and to analyze their expression in response to Zn supplementation in coffee.Coffee seedlings were cultivated in Hoagland’s solution without Zn, and after 120 days, Zn was supplied at different concentrations. Fully expanded leaves were collected for analysis of MT relative gene expression, Zn concentrations and enzymatic activity.Seven putative genes were obtained in a search of the CAFEST database. Electronic Northern analysis revealed that MTs were expressed in different tissues, developmental stages and mostly under arachidonic acid treatment. Superoxide dismutase (SOD) quantification indicated that the Zn dose influenced the enzymatic activity; the lowest being at 0.6 % Zn. Quantitative expression analyses of putative MTs demonstrated that these genes are differentially expressed in response to Zn supplementation.There is a positive correlation between MT relative expression and Zn concentration and we believe that MTs play an important role in Zn homeostasis in coffee.
Starting Page	179
Ending Page	191
Page Count	13
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	411
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-07-15
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zinc Coffee Metallothionein Plant nutrition RT-qPCR Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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