NDLI: A resin buffered method for controlling metal speciation in nutrient solutions for plant toxicity tests

Content Provider	Springer Nature Link
Author	Versieren, Liske Smets, Elke Smolders, Erik
Copyright Year	2013
Abstract	Root elongation tests are sensitive bioassays for testing metal toxicity in nutrient solutions. The metal speciation and, hence, metal exposure conditions are little controlled in the traditional set-up. A resin buffered solution system was developed to overcome this issue.Barley (Hordeum vulgare L.) root elongation was tested in aerated 140 mL solution batch systems supplied with 3.3 g Dowex resin for two plants. Copper toxicity was measured in presence or absence of the resin (+R/−R) and in presence or absence of a metal complexing ligand (+NTA; nitrilotriacetic acid/−NTA). In addition, the toxicity in the traditional set without resin and with daily solution replacement was included as a reference.Metal desorption from the resin is fast in these systems (k = 0.82 h$^{−1}$). Total dissolved Cu roughly halved during 4 days in −R/−NTA systems due to uptake, while it increased by 30 % in the +R/−NTA, probably due to complexation reactions by root-derived molecules. The toxicity (50 % reduction in root length, EC50) of the initial free Cu$^{2+}$ was equal in all resin or chelate buffered systems and in the solutions with daily replacement, whereas this threshold was significantly larger in the −R/−NTA due to Cu$^{2+}$ uptake and complexation reactions.The resin method is a convenient system for high throughput screening of metal toxicity and avoids uncertainties in metal speciation inherent to chelator buffered systems. Details are given how to prepare the resin to obtain a target metal ion activity.
Starting Page	257
Ending Page	267
Page Count	11
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	373
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-06-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metal toxicity Copper Metal buffering Cation exchange resin Plants Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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