NDLI: Cloning, characterisation and phylogenetic analysis of the sigA σ$^{70}$ factor gene sequence from the actinomycete Frankia

Content Provider	Springer Nature Link
Author	Blaha, D. Couryer, B.
Copyright Year	2003
Abstract	The σ$^{70}$ family of transcriptional factors is essential for bacterial life. Members of this family have been duplicated through evolution and have evolved and specialized for all sorts of physiological responses. Here, we have performed the first analysis of this gene family in Frankia. PCR was used to amplify Frankia σ$^{70}$ DNA regions well-conserved among primary, stationary phase and alternative actinomycetal sigma factors. DNA sequence analyses confirmed the identity of the obtained PCR fragment, which was then used to screen a Frankia ACN14a gene library. A hybridizing library clone was obtained and characterized. A 2045 bp-long DNA sequence harboring the probed region was sequenced and analysed. A single complete orf was detected. This orf has 82% identities with Streptomyces coelicolor hrdA gene sequence and its deduced protein has 59% identities and 74% similarities with the HrdA protein. A phylogenetic analysis shows the deduced protein sequence to belong to a well-resolved phylogenetic radiation containing primary σ$^{70}$ factors (essential for growth), and was thus named SigA. Early duplication events in this radiation led to the presence of several paralogs of primary-like σ$^{70}$ factors among the actinomycetes. These paralogous lineages could be divided into at least three well-resolved clusters: (A) a HrdA lineage found among Streptomyces; (B) a MysB-SigB lineage found among Mycobacterium and Brevibacterium, and (C) a MysA-HrdB lineage widespread among the actinomycetes. Frankia SigA represents a distinct lineage in this radiation. Blot analysis of digested Frankia ACN14a total DNA showed sigA to be in a single copy.
Starting Page	97
Ending Page	106
Page Count	10
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	254
Issue Number	1
e-ISSN	15735036
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecology Plant Sciences Plant Physiology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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