NDLI: A pulse-labelling method to generate 13C- enriched plant materials

Content Provider	Springer Nature Link
Author	Bromand, Søren Whalen, Joann K. Janzen, H. Henry Schjoerring, Jan K. Ellert, B.H.
Copyright Year	2001
Abstract	Plant materials labelled with $^{13}$C can be used to trace litter decomposition and root carbon flow, but only if the isotope is uniformly distributed in the plant. We postulated that if $^{13}$CO$_{2}$ were applied at regular intervals, in direct proportion to the rate of photosynthesis, then the abundance of $^{13}$C would be uniform among plant parts. To test this hypothesis, wheat plants were grown in the greenhouse, and exposed weekly to $^{13}$CO$_{2}$ for six hours in a closed chamber. A constant dose of $^{13}$CO$_{2}$ (about 33 atom%) was injected whenever CO$_{2}$ concentration fell below a prescribed limit, so that $^{13}$CO$_{2}$ was added in proportion to photosynthetic rate. Wheat exposed for 13 weeks (starting 11 days after seeding) had reasonably consistent $^{13}$C abundance among plant parts: grain = 3.41, chaff = 3.41, stem = 3.65, and root = 3.50 atom%. The `leaf' fraction had slightly higher abundance (3.99 atom%), perhaps because recently-fixed $^{13}$C was not translocated from senescing tissue. Exposing plants only during early stages of the growing season increased differences among plant parts. The approach offers a practical way to label plants with $^{13}$C.
Starting Page	253
Ending Page	257
Page Count	5
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	235
Issue Number	2
e-ISSN	15735036
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ecology Plant Sciences Plant Physiology Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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