NDLI: Molecular characterization of nitrogen fixing microsymbionts from root nodules of Vachellia (Acacia) jacquemontii, a native legume from the Thar Desert of India

Content Provider	Springer Nature Link
Author	Sankhla, Indu Singh Tak, Nisha Meghwal, Raju Ram Choudhary, Sunil Tak, Alkesh Rathi, Sonam Sprent, Janet I. James, Euan K. Gehlot, Hukam Singh
Copyright Year	2016
Abstract	To describe the structure of nodules of Vachellia (Acacia) jacquemontii, and to characterise the rhizobia that occupy them.Light and electron microscopy were used to analyse nodules. Rhizobia were characterised using their 16S rRNA, housekeeping and symbiosis-related gene sequences.Nodules of V. jacquemontii were typical of all other described mimosoid legumes. All 73 of the isolates were strains of Ensifer, and concatenated phylogenetic analysis of their housekeeping genes (rrs, recA, atpD, glnII and dnaK) suggested that they are novel, forming separate lineages close to E. saheli. The phylogenies of the symbiosis-essential genes nodA and nifH were inconsistent with the housekeeping phylogenies. The nodA sequences of most isolates were close to that of E. arboris HAMBI 1552$^{T}$, but the nifH gene was found to be related to that of E. kostiensis HAMBI 1489$^{T}$. All the tested Ensifer strains, except for AJ24, were found to be capable of nodulating other species of Vachellia as well as native Indian Mimosa and Prosopis spp.Stressful conditions caused by the alkaline soil of the Thar Desert have resulted in V. jacquemontii being nodulated by diverse and promiscuous Ensifer species that are capable of nodulating other native members of the tribe Mimoseae.
Starting Page	21
Ending Page	40
Page Count	20
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	410
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-02-29
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vachellia (Acacia) jacquemontii Thar Desert Ensifer rhizobia nifH nodA Multi Locus Sequence Analysis (MLSA) Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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