NDLI: The nectary-specific pattern of expression of the tobacco Nectarin I promoter is regulated by multiple promoter elements$^{†}$

Content Provider	Springer Nature Link
Author	Carter, Clay Thornburg, Robert W.
Copyright Year	2003
Abstract	The major protein secreted into the nectar of tobacco plants (Nectarin I) is a germin-like protein that has superoxide dismutase activity. We have isolated the gene encoding Nectarin I (NECI) and analyzed the expression of a chloramphenicol acetyl transferase (CAT) marker gene driven by its promoter in transgenic plants. Transgenic plant lines that expressed the CAT gene under control of the full-length NEC1 promoter showed high levels of CAT expression in mature floral nectaries. Tissue specificity of NECI-CAT expression demonstrated that the construct was expressed uniquely in nectaries with a small level of expression in ovary. Further, analysis of its temporal expression showed that the construct is expressed uniquely during those times when nectar is actively being secreted from flowers. An examination of the transcription start site verified that the initiation site of the NECI-CAT mRNA in transgenic plants is identical with that of the native gene in vivo. Two promoter deletion constructs were also prepared and analyzed. Analysis in transgenic plants revealed that the nectary-specific expression is the result of multiple promoter elements and suggests that nectar secretion and flower opening may be coordinately regulated.
Starting Page	451
Ending Page	457
Page Count	7
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	51
Issue Number	4
e-ISSN	15735028
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2003-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochemistry Plant Sciences Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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