NDLI: Involvement of genes encoding ABI1 protein phosphatases in the response of Brassica napus L. to drought stress

Content Provider	Springer Nature Link
Author	Babula Skowrońska, Danuta Ludwików, Agnieszka Cieśla, Agata Olejnik, Anna Cegielska Taras, Teresa Bartkowiak Broda, Iwona Sadowski, Jan
Copyright Year	2015
Abstract	In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A members. To gain insight into the conservation of ABI1 function in Brassicaceae, and understand better its regulatory effects in the drought stress response, we generated transgenic B. napus plants overexpressing A. thaliana ABI1. Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants. We present the characterization of the drought response of B. napus with the participation of ABI1-like paralogues. The expression pattern of two evolutionarily distant paralogues, BnaA01.ABI1.a and BnaC07.ABI1.b in B. napus and their promoter activity in A. thaliana showed differences in the induction of the paralogues under dehydration stress. Comparative sequence analysis of both BnaABI1 promoters showed variation in positions of cis-acting elements that are especially important for ABA- and stress-inducible expression. Together, these data reveal that subfunctionalization following gene duplication may be important in the maintenance and functional divergence of the BnaABI1 paralogues. Our results provide a framework for a better understanding of (1) the role of ABI1 as a hub protein regulator of the drought response, and (2) the differential involvement of the duplicated BnaABI1 genes in the response of B. napus to dehydration-related stresses.
Starting Page	445
Ending Page	457
Page Count	13
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	88
Issue Number	4-5
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-06-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica napus Drought response ABI1 protein phosphatase Gene overexpression Promoter activity Plant Sciences Biochemistry Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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