NDLI: Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae

Content Provider	Springer Nature Link
Author	Imamura, Sousuke Kawase, Yasuko Kobayashi, Ikki Sone, Toshiyuki Era, Atsuko Miyagishima, Shin ya Shimojima, Mie Ohta, Hiroyuki Tanaka, Kan
Copyright Year	2015
Abstract	Most microalgae produce triacylglycerol (TAG) under stress conditions such as nitrogen depletion, but the underlying molecular mechanism remains unclear. In this study, we focused on the role of target of rapamycin (TOR) in TAG accumulation. TOR is a serine/threonine protein kinase that is highly conserved and plays pivotal roles in nitrogen and other signaling pathways in eukaryotes. We previously constructed a rapamycin-susceptible Cyanidioschyzon merolae, a unicellular red alga, by expressing yeast FKBP12 protein to evaluate the results of TOR inhibition (Imamura et al. in Biochem Biophys Res Commun 439:264–269, 2013). By using this strain, we here report that rapamycin-induced TOR inhibition results in accumulation of cytoplasmic lipid droplets containing TAG. Transcripts for TAG synthesis-related genes, such as glycerol-3-phosphate acyltransferase and acyl-CoA:diacylglycerol acyltransferase (DGAT), were increased by rapamycin treatment. We also found that fatty acid synthase-dependent de novo fatty acid synthesis was required for the accumulation of lipid droplets. Induction of TAG and up-regulation of DGAT gene expression by rapamycin were similarly observed in the unicellular green alga, Chlamydomonas reinhardtii. These results suggest the general involvement of TOR signaling in TAG accumulation in divergent microalgae.
Starting Page	309
Ending Page	318
Page Count	10
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	89
Issue Number	3
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chlamydomonas reinhardtii Cyanidioschyzon merolae Lipid droplet Microalga Target of rapamycin Triacylglycerol Plant Sciences Biochemistry Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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