NDLI: P19-dependent and P19-independent reversion of F1-V gene silencing in tomato

Content Provider	Springer Nature Link
Author	Alvarez, M. Lucrecia Pinyerd, Heidi L. Topal, Emel Cardineau, Guy A.
Copyright Year	2008
Abstract	As a part of a project to develop a plant-made plague vaccine, we expressed the Yersinia pestis F1-V antigen fusion protein in tomato. We discovered that in some of these plants the expression of the f1-v gene was undetectable in leaves and fruit by ELISA, even though they had multiple copies of f1-v according to Southern-blot analysis. A likely explanation of these results is the phenomenon of RNA silencing, a group of RNA-based processes that produces sequence-specific inhibition of gene expression and may result in transgene silencing in plants. Here we report the reversion of the f1-v gene silencing in transgenic tomato plants through two different mechanisms. In the P19-dependent Reversion or Type I, the viral suppressor of gene silencing, P19, induces the reversion of gene silencing. In the P19-independent Reversion or Type II, the f1-v gene expression is restored after the substantial loss of gene copies as a consequence of transgene segregation in the progeny. The transient and stable expression of the p19 gene driven by a constitutive promoter as well as an ethanol inducible promoter induced a P19-dependent reversion of f1-v gene silencing. In particular, the second generation plant 3D1.6 had the highest P19 protein levels and correlated with the highest F1-V protein accumulation, almost a three-fold increase of F1-V protein levels in fruit than that previously reported for the non-silenced F1-V elite tomato lines. These results confirm the potential exploitation of P19 to substantially increase the expression of value-added proteins in plants.
Starting Page	61
Ending Page	79
Page Count	19
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	68
Issue Number	1-2
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-06-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	P19 PTGS RNA silencing Tomato Transgenic plants Viral suppressor Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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