NDLI: Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture

Content Provider	Springer Nature Link
Author	Hong, Shin Young Lee, Tae Sup Kim, Ju Jung, Jae Ho Choi, Chang Woon Kim, Tae Geum Kwon, Tae Ho Jang, Yong Suk Yang, Moon Sik
Copyright Year	2008
Abstract	The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we developed a transgenic rice cell suspension culture system. The in vivo assembly and secretion of hzAb were achieved in a transgenic rice cell culture under the control of the rice alpha amylase 3D (RAmy 3D) expression system, and the biological activities of plant-derived hzAb were determined to be quite similar to those of animal-derived antibody. Purified hzAb was shown to bind to the recombinant antigen, TAG 72, and to bind specifically to human LS 174T colon adenocarcinoma cells expressing the TAG 72 antigen, and this binding occurred to the same extent as was seen with animal-derived antibody. Plant-derived hzAb proved as effective as animal-derived antibody in targeting tumors of xenotransplanted LS 174T cells in nude mice. The results of this study indicate that the hzAb derived from plant cell suspension cultures may have great potential for pharmaceutical applications in the development of future cancer therapeutic and diagnostic protocols.
Starting Page	413
Ending Page	422
Page Count	10
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	68
Issue Number	4-5
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-08-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	hzAb RAmy 3D TAG 72 Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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