NDLI: G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

Content Provider	Springer Nature Link
Author	Chakraborty, Navjyoti Sharma, Priyanka Kanyuka, Kostya Pathak, Ravi Ramesh Choudhury, Devapriya Hooley, Richard Raghuram, Nandula
Copyright Year	2015
Abstract	Heterotrimeric G-proteins are implicated in several plant processes, but the mechanisms of signal-response coupling and the roles of G-protein coupled receptors in general and GCR1 in particular, remain poorly understood. We isolated a knock-out mutant of the Arabidopsis G-protein α subunit (gpa1-5) and analysed its transcriptome to understand the genomewide role of GPA1 and compared it with that of our similar analysis of a GCR1 mutant (Chakraborty et al. 2015, PLoS ONE 10(2):e0117819). We found 394 GPA1-regulated genes spanning 79 biological processes, including biotic and abiotic stresses, development, flavonoid biosynthesis, transcription factors, transporters and nitrate/phosphate responses. Many of them are either unknown or unclaimed explicitly in other published gpa1 mutant transcriptome analyses. A comparison of all known GPA1-regulated genes (including the above 394) with 350 GCR1-regulated genes revealed 114 common genes. This can be best explained by GCR1–GPA1 coupling, or by convergence of their independent signaling pathways. Though the common genes in our GPA1 and GCR1 mutant datasets constitute only 26 % of the GPA1-regulated and 30 % of the GCR1-responsive genes, they belong to nearly half of all the processes affected in both the mutants. Thus, GCR1 and GPA1 regulate not only some common genes, but also different genes belonging to the same processes to achieve similar outcomes. Overall, we validate some known and report many hitherto unknown roles of GPA1 in plants, including agronomically important ones such as biotic stress and nutrient response, and also provide compelling genetic evidence to revisit the role of GCR1 in G-protein signalling.
Starting Page	559
Ending Page	576
Page Count	18
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	89
Issue Number	6
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis thaliana G-protein Gα GPA1 GPCR GCR1 Development Flavonoid Nutrient Stress Transcriptome Functional genomics Microarray Plant Sciences Biochemistry Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

G-protein Signaling Components GCR1 and GPA1 Mediate Responses to Multiple Abiotic Stresses in Arabidopsis

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein α-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development

Heterotrimeric G protein α subunit (GPA1) regulates the response to low-nitrogen stress in Arabidopsis by interacting with AtNRT1.4 and AtATG8a

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

G-protein Signaling Components GCR1 and GPA1 Mediate Responses to Multiple Abiotic Stresses in Arabidopsis

Flavonoid diversity and biosynthesis in seed of Arabidopsis thaliana

Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana.

The α-Subunit of the Arabidopsis Heterotrimeric G Protein, GPA1, Is a Regulator of Transpiration Efficiency

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana

Similar Documents

G-protein Signaling Components GCR1 and GPA1 Mediate Responses to Multiple Abiotic Stresses in Arabidopsis

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein α-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development

Heterotrimeric G protein α subunit (GPA1) regulates the response to low-nitrogen stress in Arabidopsis by interacting with AtNRT1.4 and AtATG8a

The Arabidopsis Putative G Protein–Coupled Receptor GCR1 Interacts with the G Protein α Subunit GPA1 and Regulates Abscisic Acid Signaling

G-protein Signaling Components GCR1 and GPA1 Mediate Responses to Multiple Abiotic Stresses in Arabidopsis

Flavonoid diversity and biosynthesis in seed of Arabidopsis thaliana

Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana.

The α-Subunit of the Arabidopsis Heterotrimeric G Protein, GPA1, Is a Regulator of Transpiration Efficiency

G-protein α-subunit (GPA1) regulates stress, nitrate and phosphate response, flavonoid biosynthesis, fruit/seed development and substantially shares GCR1 regulation in A. thaliana