NDLI: Roles of the different components of magnesium chelatase in abscisic acid signal transduction

Content Provider	Springer Nature Link
Author	Du, Shu Yuan Zhang, Xiao Feng Lu, Zekuan Xin, Qi Wu, Zhen Jiang, Tao Lu, Yan Wang, Xiao Fang Zhang, Da Peng
Copyright Year	2012
Abstract	The H subunit of Mg-chelatase (CHLH) was shown to regulate abscisic acid (ABA) signaling and the I subunit (CHLI) was also reported to modulate ABA signaling in guard cells. However, it remains essentially unknown whether and how the Mg-chelatase-catalyzed Mg-protoporphyrin IX-production differs from ABA signaling. Using a newly-developed surface plasmon resonance system, we showed that ABA binds to CHLH, but not to the other Mg-chelatase components/subunits CHLI, CHLD (D subunit) and GUN4. A new rtl1 mutant allele of the CHLH gene in Arabidopsis thaliana showed ABA-insensitive phenotypes in both stomatal movement and seed germination. Upregulation of CHLI1 resulted in ABA hypersensitivity in seed germination, while downregulation of CHLI conferred ABA insensitivity in stomatal response in Arabidopsis. We showed that CHLH and CHLI, but not CHLD, regulate stomatal sensitivity to ABA in tobacco (Nicotiana benthamiana). The overexpression lines of the CHLD gene showed wild-type ABA sensitivity in Arabidopsis. Both the GUN4-RNA interference and overexpression lines of Arabidopsis showed wild-type phenotypes in the major ABA responses. These findings provide clear evidence that the Mg-chelatase-catalyzed Mg-ProtoIX production is distinct from ABA signaling, giving information to understand the mechanism by which the two cellular processes differs at the molecular level.
Starting Page	519
Ending Page	537
Page Count	19
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	80
Issue Number	4-5
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-09-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	H subunit of Mg-chelatase I subunit of Mg-chelatase D subunit of Mg-chelatase GUN4 ABA binding ABA signal transduction Biochemistry Plant Pathology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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Roles of the different components of magnesium chelatase in abscisic acid signal transduction

Roles of the different components of magnesium chelatase in abscisic acid signal transduction

Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana

Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana

Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis

Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis

Expression analysis of the cDNA for magnesium chelatase H subunit (CHLH) during sweet cherry fruit ripening and under stress conditions

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Roles of the different components of magnesium chelatase in abscisic acid signal transduction

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