NDLI: Direct Interaction between the Tobacco Mosaic Virus Helicase Domain and the ATP-bound Resistance Protein, N Factor during the Hypersensitive Response in Tobacco Plants

Content Provider	Springer Nature Link
Author	Ueda, Hirokazu Yamaguchi, Yube Sa, Hiroshi
Copyright Year	2006
Abstract	Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we analyzed the mode of interaction between the N factor, a tobacco R factor, and the helicase domain (p50) of tobacco mosaic virus (TMV). To this end, domain dissected proteins were prepared and subjected to Agroinfiltration into intact leaves, followed by yeast two hybrid and pull-down assays. The results pointed to three novel features. First, the N factor was found to directly bind to the p50 of TMV, second, ATP was pre-requisite for this interaction, with formation of an ATP/N factor complex, and third, the N factor was shown to possess ATPase activity, which is enhanced by the p50. Moreover, we found that intra- and/or inter-molecular interactions take place in the N factor molecule. This interaction required ATP, and was disrupted by the p50. Based on these results, we propose a following model for the TMV recognition mechanism in tobacco plants. The N factor forms a complex with ATP, to which the helicase domain interacts, and enhances ATP hydrolysis. The resulting ADP/N factor complex then changes its conformation, thereby facilitating further interaction with the down-stream signaling factor(s). This model is consistent with the idea of ‘protein machine’.
Starting Page	31
Ending Page	45
Page Count	15
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	61
Issue Number	1-2
e-ISSN	15735028
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ATP helicase hypersensitive response N factor Nicotiana tabacum tobacco mosaic virus Biochemistry Plant Sciences Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants

Members of 14-3-3 protein isoforms interacting with the resistance gene product N and the elicitor of Tobacco mosaic virus

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Direct Interaction between the Tobacco Mosaic Virus Helicase Domain and the ATP-bound Resistance Protein, N Factor during the Hypersensitive Response in Tobacco Plants

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