NDLI: Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence

Content Provider	Springer Nature Link
Author	Ay, Nicole Janack, Bianka Fischer, Andreas Reuter, Gunter Humbeck, Klaus
Copyright Year	2015
Abstract	The barley gene HvS40, encoding a putative regulator of leaf senescence, is strongly induced during leaf senescence. As shown by chromatin immunoprecipitation, euchromatic histone modification H3K9ac is added at promoter close to ATG and coding sequence of HvS40 after onset of senescence. In parallel, level of heterochromatic H3K9me2 decreases at this gene. Bisulfite sequencing revealed no DNA-methylation in this region, but a heavily methylated DNA-island, starting 664 bp upstream from translational start site in both, mature and senescent leaves. A decrease in DNA methylation in senescing leaves could be shown at one specific CpG motif at the end of this methylation island. In addition, global changes in chromatin structure during senescence were analyzed via immunocytology, revealing senescence-associated changes in spatial distribution of heterochromatic H3K9me2 patterns in the nuclei. Our results prove a senescence-specific mechanism, altering histone modification marks at distinct sequences of the senescence-associated gene HvS40 and altering distribution of heterochromatic areas in the nuclei.
Starting Page	127
Ending Page	141
Page Count	15
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	89
Issue Number	1-2
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-08-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA methylation Epigenetic Histone modifications Hordeum vulgare HvS40 Leaf senescence Plant Sciences Biochemistry Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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