NDLI: Transcript profiling of the hypomethylated hog1 mutant of Arabidopsis

Content Provider	Springer Nature Link
Author	Jordan, Nicholas David West, Jonathan Paul Bottley, Andrew Sheikh, Mazhar Furner, Ian
Copyright Year	2007
Abstract	Transcript profiling was used to look for genes that differ in expression between the SAH hydrolase deficient and hypomethylated hog1-1 mutant and the parental (HOG1) line. This analysis identified a subset of gene transcripts that were up-regulated in hog1-1 plants. The majority of these transcripts were from genes located in the pericentromeric heterochromatin. About a third of the genes are annotated as transposons or having transposon homology. Subsequent experiments using Northern blots, RT-PCR and real-time RT-PCR confirmed the up-regulation of 19 of the genes and identified a set of molecular probes for genes that are up-regulated in the hog1-1 background. Six (of six genes tested) of the hog1-1 up-regulated genes are also up-regulated in the hypomethylated ddm1 mutant, three in the hypomethylated met1 mutant and three in the dcl3 mutant. The results suggest that the hypomethylation in the mutant lines may have a causal role in the up-regulation of these transcripts.
Starting Page	571
Ending Page	586
Page Count	16
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	65
Issue Number	5
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-09-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Methylation Centromere Heterochromatin Transposon Transcript Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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