NDLI: High Irradiance Effects on the Xanthophyll Cycle Pigments and the Activity of Violaxanthin De-Epoxidase in Soybean Callus

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High Irradiance Effects on the Xanthophyll Cycle Pigments and the Activity of Violaxanthin De-Epoxidase in Soybean Callus

Content Provider	Springer Nature Link
Author	Pandey, D.M. Kim, K.H. Kang, K.H. Yeo, U.D.
Copyright Year	2004
Abstract	High irradiance (HI) effects on xanthophyll cycle pigments (XCP) and activity of violaxanthin de-epoxidase (VDE) in terms of de-epoxidation index (DEI) were studied in soybean calli. The calli from the hypocotyl segments of 5-d seedlings were induced on a solid (1.1 % agar) MS medium (pH 5.8) supplemented with 4.52 μM 2,4-dichloro-phenoxyacetic acid, 2.32 μM kinetin, and 3 % sucrose. After a 30 d cultivation, the green calli were irradiated for 24 h with “white light” (HI, 1 300 μmol m$^{−2}$ s$^{−1}$) and VDE was isolated from the photosystem 2 (PS2) particles. In the control (0 h irradiation) callus, the reaction of PS2 particles with VDE in the presence or absence of Tween 20 resulted in the decrease of VIO content and the increase of ZEA content. In the 24 h HI-callus, the reaction of PS2 particles in the absence of VDE led to the decrease of VIO and ANT contents and increase of ZEA content. In the control, DEIs in the presence of VDE with or without 0.1 %Tween 20 (1.04 and 1.06, respectively) were significantly higher than the DEI (0.76) in the absence of VDE. In the HI-callus, DEIs in the presence of VDE with or without 0.1 %Tween 20 (0.98 and 0.96, respectively) were similar to that (1.03) in the absence of VDE.
Starting Page	153
Ending Page	156
Page Count	4
File Format	PDF
ISSN	03003604
Journal	Photosynthetica
Volume Number	42
Issue Number	1
e-ISSN	15739058
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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